Caspase-3 Protein Overview: Sequence, Structure, Function and Protein Interaction

Caspase-3 Protein Overview

Cysteinyl aspartate-specific proteases, or caspases, such as CASP3, cleave substrates directly after an aspartic acid residue and play essential roles in programmed cell death. Caspases are synthesized in a dormant form with an N-terminal prodomain followed by a large subunit and a small subunit. Proteolytic processing releases the caspase large and small subunits, resulting in activation (summary by Parker et al., 2010).

Caspase-3 protein family

Belongs to the peptidase C14A family.

Caspase-3 protein name

Recommended name
Caspase-3
Short name
CASP-3
Aliases
apopain, CPP32, CPP32B, Yama
Alternative name
Apopain Cysteine protease CPP32 CPP-32 Protein Yama SREBP cleavage activity 1 SCA-1) Caspase-3 subunit p17 Caspase-3 subunit p12

Caspase-3 Gene family protein

Caspases

Caspase-3 Protein Sequence

Species Human Caspase-3 protein
Length 277
Mass (Da) 31608
Sequence Human Caspase-3 protein sequence
Species Mouse Caspase-3 protein
Length 277
Mass (Da) 31475
Sequence Mouse Caspase-3 protein sequence
Species Rat Caspase-3 protein
Length 277
Mass (Da) 31492
Sequence Rat Caspase-3 protein sequence

Caspase-3 Protein Molecular Weight & PI

Caspase-3 precursor (EC 3.4.22.56) (CASP-3) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Contains: Caspase-3 subunit p17; Caspase-3 subunit p12] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 29-175 Caspase-3 subunit p17.

Molecular weight (Da)

16614.85

Theoretical pI

6.80

Caspase-3 Protein Structure

CRYSTAL STRUCTURE OF THE COMPLEX OF APOPAIN WITH THE TETRAPEPTIDE INHIBITOR ACE-DVAD-FMC
Deposited
1996-12-12   Released:  1997-12-24
Deposition Author(s)
Mittl, P.R.E., Dimarco, S., Gruetter, M.G.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.3000 Å
R-Value Free
0.284
R-Value Work
0.188
1CP3 From PDB

Human Caspase-3 protein Secondary structure

Caspase-3 Protein Interaction

Recombinant Caspase-3 Protein Feature

Caspase-3 Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.
High Activity
Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is > 5000 pmoles/min/μg.

Recombinant Caspase-3 protein citations

Title
Sulforaphane-cysteine-induced apoptosis via phosphorylated ERK1/2-mediated maspin pathway in human non-small cell lung cancer cells
Year
2017
Author
Lin, K;Yang, R;Zheng, Z;Zhou, Y;Geng, Y;Hu, Y;Wu, S;Wu, W;
Journal
Cell Death Discov
Title
WT1 protein is cleaved by caspase-3 in apoptotic leukemic cells
Year
2017
Author
Ruan, J;Gao, S;Yang, J;Li, H;Huang, H;Zheng, X;
Journal
Leuk. Lymphoma
Title
Sulforaphane metabolites reduce resistance to paclitaxel via microtubule disruption
Year
2018
Author
Wang, Y;Zhou, Y;Zheng, Z;Li, J;Yan, Y;Wu, W;
Journal
Cell Death Dis
Title
Apoptotic Caspases Suppress Type I Interferon Production via the Cleavage of cGAS, MAVS, and IRF3
Year
2019
Author
Ning, X;Wang, Y;Jing, M;Sha, M;Lv, M;Gao, P;Zhang, R;Huang, X;Feng, JM;Jiang, Z;
Journal
Mol. Cell
Title
Time-resolved protein activation by proximal decaging in living systems
Year
2019
Author
Wang, J;Liu, Y;Liu, Y;Zheng, S;Wang, X;Zhao, J;Yang, F;Zhang, G;Wang, C;Chen, PR;
Journal
Nature
Title
Peptide Microarray-Based Fluorescence Assay for Quantitatively Monitoring the Tumor-Associated Matrix Metalloproteinase-2 Activity
Year
2019
Author
Jian, M;Su, M;Gao, J;Wang, Z;
Journal
Sensors and Actuators B: Chemical

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