CD200R Antibody Guideline: Preparation, Dilution and Applications

CD200R Antibody Overview

There are 18 CD200R antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB, IP, ELISA(Det), ELISA(Cap). There are 11 CD200R antibody for ELISA, 6 CD200R antibody for FCM, 3 CD200R antibody for WB, 1 CD200R antibody for IP, 1 CD200R antibody for ELISA(Det), 1 CD200R antibody for ELISA(Cap). Among all these CD200R antibodies, there are 6 anti-CD200R mouse monoclonal antibodies , 5 anti-CD200R rabbit monoclonal antibodies , 7 anti-CD200R rabbit polyclonal antibodies . All the CD200R anbodies are produced in house and all are in stock. CD200R antibody customerized service is available.

CD200R Antibody ELISA

CD200R antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD200R1 (rh CD200R1; Catalog#11218-H08H; AAI43394.1; Met1-Leu266).

CD200R antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

CD200R Antibody western blot

CD200R Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD200R1 extracellular domain (rh CD200R1; Catalog#11218-H08H; AAI43394.1; Met 1-Leu 266). Total IgG was purified by Protein A affinity chromatography.

CD200R Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD200R Antibody flow cytometry

CD200R Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD200R1 (rh CD200R1; Catalog#11218-H08H; AAI43394.1; Met1-Leu266). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD200R Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD200R Antibody immunoprecipitation

CD200R Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD200R1 (rh CD200R1; Catalog#11218-H08H; AAI43394.1; Met1-Leu266). CD200R1 specific IgG was purified by Human CD200R1 affinity chromatography.

CD200R Antibody immunoprecipitation Validated

Application IP
Dilution 1-2 μL/mg of lysate
Notes  

CD200R antibody citations

Title
Evaluation of soluble CD200 levels in type 2 diabetic foot and nephropathic patients: association with disease activity
Year
2014
Author
Arik, HO;Yalcin, AD;Celik, B;Seyman, D;Tetik, G;Gursoy, B;Kose, S;Gumuslu, S;
Journal
Med. Sci. Monit.