CD21 Antibody Guideline: Preparation, Dilution and Applications

CD21 Antibody Overview

There are 17 CD21 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ELISA(Cap), IHC-P, ELISA(Det), WB, IP. There are 6 CD21 antibody for ELISA, 8 CD21 antibody for FCM, 2 CD21 antibody for ELISA(Cap), 4 CD21 antibody for IHC-P, 1 CD21 antibody for ELISA(Det), 2 CD21 antibody for WB, 1 CD21 antibody for IP. Among all these CD21 antibodies, there are 6 anti-CD21 mouse monoclonal antibodies , 7 anti-CD21 rabbit monoclonal antibodies , 4 anti-CD21 rabbit polyclonal antibodies . All the CD21 anbodies are produced in house and all are in stock. CD21 antibody customerized service is available.

CD21 Antibody ELISA

CD21 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD21 (rh CD21; Catalog#10811-H08H; P20023-1; Met 1-Arg 971). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD21 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD21 Antibody western blot

CD21 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD21 (rh CD21; Catalog#10811-H08H; P20023-1; Met 1-Arg 971). CD21 specific IgG was purified by Human CD21 affinity chromatography.

CD21 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD21 Antibody flow cytometry

CD21 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD21 (rh CD21; Catalog#10811-H08H; P20023-1; Met1-Arg971). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD21 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD21 Antibody immunohistochemistry

CD21 Antibody immunoprecipitation

CD21 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD21 (rh CD21; Catalog#10811-H08H; P20023-1; Met1-Arg971). CD21 specific IgG was purified by Human CD21 affinity chromatography.

CD21 Antibody immunoprecipitation Validated

Application IP
Dilution IP:0.5-2 μL/mg of lysate
Notes