CD31 Antibody Guideline: Preparation, Dilution and Applications

CD31 Antibody Overview

There are 26 CD31 antibodies which are validated in multiple tissues with various applications, including FCM, ELISA(Cap), ELISA(Det), ICC/IF, IHC-P, ELISA, WB, IP. There are 13 CD31 antibody for FCM, 1 CD31 antibody for ELISA(Cap), 1 CD31 antibody for ELISA(Det), 3 CD31 antibody for ICC/IF, 8 CD31 antibody for IHC-P, 7 CD31 antibody for ELISA, 2 CD31 antibody for WB, 2 CD31 antibody for IP. Among all these CD31 antibodies, there are 13 anti-CD31 mouse monoclonal antibodies , 9 anti-CD31 rabbit monoclonal antibodies , 4 anti-CD31 rabbit polyclonal antibodies . All the CD31 anbodies are produced in house and all are in stock. CD31 antibody customerized service is available.

CD31 gene family antibody

CD31 Antibody ELISA

CD31 Antibody western blot

CD31 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD31/PECAM1 (rh CD31/PECAM1; Catalog#10148-H08H; EAW94208.1; Met1-Lys601). CD31/PECAM1 specific IgG was purified by Human CD31/PECAM1 affinity chromatography.

CD31 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD31 Antibody flow cytometry

CD31 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM-1 (rh CD31 / PECAM-1; Catalog#10148-H08H; EAW94208.1; Met1-Lys601). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD31 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD31 Antibody immunohistochemistry

CD31 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD31 / PECAM1 (rh CD31 / PECAM1; Catalog#10148-H08H; EAW94208.1; Met 1-Lys 601).

CD31 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

CD31 Antibody immunoprecipitation

CD31 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD31/PECAM-1 (rh CD31/PECAM-1; Catalog#10148-H08H; EAW94208.1; Met1-Lys601). CD31/PECAM-1 specific IgG was purified by Human CD31/PECAM-1 affinity chromatography.

CD31 Antibody immunoprecipitation Validated

Application IP
Dilution 5-10 μL/mg of lysate
Notes  

CD31 Antibody ICC / IF

CD31 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 extracellular domain (rhCD31; Catalog#10148-H08H; Met 1-Lys 601; NP_000433.3). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD31 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD31 antibody citations

Title
Barbigerone, an isoflavone, inhibits tumor angiogenesis and human non-small-cell lung cancer xenografts growth through VEGFR2 signaling pathways
Year
2012
Author
Li, X;Wang, X;Ye, H;Peng, A;Chen, L;
Journal
Cancer Chemother. Pharmacol.
Title
Gambogic acid exhibits anti-psoriatic efficacy through inhibition of angiogenesis and inflammation
Year
2014
Author
Wen, J;Pei, H;Wang, X;Xie, C;Li, S;Huang, L;Qiu, N;Wang, W;Cheng, X;Chen, L;
Journal
J. Dermatol. Sci.
Title
2, 5-Dihydro-2, 5-dimethoxyfuran crosslinked silk-chitosan blend tubular construct for vascular graft application
Year
2016
Author
Pawar, HS;Francis, NK;Rameshbabu, AP;Dhara, S;
Journal
Materials Today Communications