CD32B/Fcgr2b Antibody Guideline: Preparation, Dilution and Applications

CD32B/Fcgr2b Antibody Overview

There are 22 CD32B/Fcgr2b antibodies which are validated in multiple tissues with various applications, including ELISA(Cap), ELISA, WB, IHC-P, ELISA(Det), FCM. There are 1 CD32B/Fcgr2b antibody for ELISA(Cap), 12 CD32B/Fcgr2b antibody for ELISA, 2 CD32B/Fcgr2b antibody for WB, 4 CD32B/Fcgr2b antibody for IHC-P, 1 CD32B/Fcgr2b antibody for ELISA(Det), 8 CD32B/Fcgr2b antibody for FCM. Among all these CD32B/Fcgr2b antibodies, there are 8 anti-CD32B/Fcgr2b mouse monoclonal antibodies , 7 anti-CD32B/Fcgr2b rabbit monoclonal antibodies , 7 anti-CD32B/Fcgr2b rabbit polyclonal antibodies . All the CD32B/Fcgr2b anbodies are produced in house and all are in stock. CD32B/Fcgr2b antibody customerized service is available.

CD32B/Fcgr2b Antibody ELISA

CD32B/Fcgr2b antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Rat CD32b / Fc gamma RIIB (rR CD32b / Fc gamma RIIB; Catalog#80018-R08H; Q63203-1; Met 1-Pro 212). CD32b / Fc gamma RIIB specific IgG was purified by Rat CD32b / Fc gamma RIIB affinity chromatography.

CD32B/Fcgr2b antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

CD32B/Fcgr2b Antibody western blot

CD32B/Fcgr2b Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Rat CD32b / Fc gamma RIIB (rR CD32b / Fc gamma RIIB; Catalog#80018-R08H; Q63203-1; Met 1-Pro 212). Total IgG was purified by Protein A affinity chromatography.

CD32B/Fcgr2b Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD32B/Fcgr2b Antibody flow cytometry

CD32B/Fcgr2b Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus CD32b / Fc gamma RIIB (Catalog#90014-C08H; AAL92097.1; Met1-Pro217).

CD32B/Fcgr2b Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD32B/Fcgr2b Antibody immunohistochemistry

CD32B/Fcgr2b Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus CD32b / FCGR2B / Fc gamma RIIB ( Catalog#90014-C08H; AAL92097.1; Met1-Pro217). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD32B/Fcgr2b Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD32B/Fcgr2b antibody citations

Title
Fusion proteins for the treatment of allergic diseases
Year
2012
Author
MMBW Dooper, B Bogen, HR Myrset
Journal
US 20140120123 A1
Title
Fusion proteins for the treatment of allergic diseases
Year
2012
Author
MMBW Dooper, B Bogen, HR Myrset
Journal
US 20140120123 A1
Title
The expression of Fcγ receptors in Hashimoto's thyroiditis
Year
2015
Author
Liu, Y;Liu, M;Zhang, Y;Qu, C;Lu, G;Huang, Y;Zhang, H;Yu, N;Yuan, S;Gao, Y;Gao, Y;Guo, X;
Journal
Cell. Immunol.
Title
The expression of Fcγ receptors in Hashimoto's thyroiditis
Year
2015
Author
Liu, Y;Liu, M;Zhang, Y;Qu, C;Lu, G;Huang, Y;Zhang, H;Yu, N;Yuan, S;Gao, Y;Gao, Y;Guo, X;
Journal
Cell. Immunol.
Title
IL-3 up-regulates and activates human eosinophil CD32 and αMβ2 integrin causing degranulation
Year
2016
Author
Esnault, S;Johansson, MW;Kelly, EA;Koenderman, L;Mosher, DF;Jarjour, NN;
Journal
Clin. Exp. Allergy