CD50 Antibody Guideline: Preparation, Dilution and Applications

CD50 Antibody Overview

There are 11 CD50 antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA(Cap), FCM, ELISA, WB, IP. There are 3 CD50 antibody for IHC-P, 1 CD50 antibody for ELISA(Cap), 4 CD50 antibody for FCM, 4 CD50 antibody for ELISA, 1 CD50 antibody for WB, 1 CD50 antibody for IP. Among all these CD50 antibodies, there are 6 anti-CD50 mouse monoclonal antibodies , 3 anti-CD50 rabbit monoclonal antibodies , 2 anti-CD50 rabbit polyclonal antibodies . All the CD50 anbodies are produced in house and all are in stock. CD50 antibody customerized service is available.

CD50 gene family antibody

CD50 Antibody ELISA

CD50 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM3 (rh ICAM3; Catalog#10333-H08H; CAA49369.1; Met 1-His 485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD50 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD50 Antibody western blot

CD50 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human ICAM3 / CD50 (rh ICAM3 / CD50; Catalog#10333-H08H; CAA49369.1; Met 1-His 485). ICAM3 / CD50 specific IgG was purified by Human ICAM3 / CD50 affinity chromatography.

CD50 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD50 Antibody flow cytometry

CD50 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD50 / ICAM-3 (rh CD50 / ICAM-3; Catalog#10333-H08H; CAA49369.1; Met1-His485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD50 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD50 Antibody immunohistochemistry

CD50 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD50 / ICAM-3 (rh CD50 / ICAM-3; Catalog#10333-H08H; CAA49369.1; Met1-His485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD50 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD50 Antibody immunoprecipitation

CD50 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human ICAM3 / CD50 (rh ICAM3 / CD50; Catalog#10333-H08H; CAA49369.1; Met 1-His 485). ICAM3 / CD50 specific IgG was purified by Human ICAM3 / CD50 affinity chromatography.

CD50 Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

CD50 antibody citations

Title
Estradiol Promotes Breast Cancer Cell Migration via Recruitment and Activation of Neutrophils
Year
2017
Author
Vazquez Rodriguez, G;Abrahamsson, A;Jensen, LD;Dabrosin, C;
Journal
Cancer Immunol Res