CD68 Antibody Guideline: Preparation, Dilution and Applications

CD68 Antibody Overview

There are 12 CD68 antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA, ICC/IF, WB. There are 8 CD68 antibody for IHC-P, 8 CD68 antibody for ELISA, 1 CD68 antibody for ICC/IF, 1 CD68 antibody for WB. Among all these CD68 antibodies, there are 3 anti-CD68 mouse monoclonal antibodies , 4 anti-CD68 rabbit monoclonal antibodies , 5 anti-CD68 rabbit polyclonal antibodies . All the CD68 anbodies are produced in house and all are in stock. CD68 antibody customerized service is available.

CD68 Antibody ELISA

CD68 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD68 (rh CD68; Catalog#11192-H08B1; P34810-1; Met1-Ser319). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD68 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD68 Antibody western blot

CD68 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD68 (rh CD68; Catalog#11192-H08B1; P34810-1; Met1-Ser319). CD68 specific IgG was purified by Human CD68 affinity chromatography.

CD68 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD68 Antibody immunohistochemistry

CD68 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD68 (rh CD68; Catalog#11192-H08B1; P34810-1; Met1-Ser319). CD68 specific IgG was purified by Human CD68 affinity chromatography.

CD68 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:2500-1:10000
Notes  

CD68 antibody citations

Title
Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages
Year
2020
Author
Trimova, G;Yamagata, K;Iwata, S;Hirata, S;Zhang, T;Uemura, F;Satoh, M;Biln, N;Nakayamada, S;Maksymowych, WP;Tanaka, Y;
Journal
Arthritis Res. Ther.