CD8 alpha Antibody Guideline: Preparation, Dilution and Applications

CD8 alpha Antibody Overview

There are 43 CD8 alpha antibodies which are validated in multiple tissues with various applications, including ELISA(Cap), ELISA, ELISA(Det), FCM, IHC-P, WB, ICC/IF. There are 3 CD8 alpha antibody for ELISA(Cap), 14 CD8 alpha antibody for ELISA, 2 CD8 alpha antibody for ELISA(Det), 22 CD8 alpha antibody for FCM, 6 CD8 alpha antibody for IHC-P, 1 CD8 alpha antibody for WB, 2 CD8 alpha antibody for ICC/IF. Among all these CD8 alpha antibodies, there are 16 anti-CD8 alpha mouse monoclonal antibodies , 19 anti-CD8 alpha rabbit monoclonal antibodies , 8 anti-CD8 alpha rabbit polyclonal antibodies . All the CD8 alpha anbodies are produced in house and all are in stock. CD8 alpha antibody customerized service is available.

CD8 alpha Antibody ELISA

CD8 alpha Antibody western blot

CD8 alpha Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD8a / Lyt2 (rh CD8a / Lyt2; Catalog#10980-H08H; NP_001759.3; Met1-Asp182). CD8a / Lyt2 specific IgG was purified by Human CD8a / Lyt2 affinity chromatography.

CD8 alpha Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD8 alpha Antibody flow cytometry

CD8 alpha Antibody immunohistochemistry

CD8 alpha Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD8/CD8 alpha/Leu-2 (rh CD8/CD8 alpha/Leu-2; Catalog#10980-H08H; NP_001759.3; Met1-Asp182). CD8/CD8 alpha/Leu-2 specific IgG was purified by Human CD8/CD8 alpha/Leu-2 affinity chromatography.

CD8 alpha Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CD8 alpha Antibody ICC / IF

CD8 alpha Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD8a / Lyt2 (rM CD8a / Lyt2; Catalog#50389-M08H; NP_001074579.1; Met1-Tyr196).

CD8 alpha Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD8 alpha antibody citations

Title
Heterogeneous pathological outcomes after experimental pH1N1 influenza infection in ferrets correlate with viral replication and host immune responses in the lung
Year
2014
Author
Vidaña, B;Martínez, J;Martínez-Orellana, P;García Migura, L;Montoya, M;Martorell, J;Majó, N;
Journal
Vet. Res.
Title
Pathogenesis of 2009 pandemic influenza a virus (H1N1) infection in the ferret model
Year
2015
Author
Vidaña Mateo, B;
Journal
Thesis
Title
Peripheral Leukocyte Migration in Ferrets in Response to Infection with Seasonal Influenza Virus
Year
2016
Author
Music, N;Reber, AJ;Kim, JH;York, IA;
Journal
PLoS ONE
Title
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Year
2017
Author
Sung, SJ;Ge, Y;Dai, C;Wang, H;Fu, SM;Sharma, R;Hahn, YS;Yu, J;Le, TH;Okusa, MD;Bolton, WK;Lawler, JR;
Journal
J. Immunol
Title
The targeted delivery of interleukin-12 to the carcinoembryonic antigen increases the intratumoral density of NK and CD8+ T cell in an immunocompetent mouse model of colorectal cancer
Year
2020
Author
Puca, E;Schmitt-Koopmann, C;Furter, M;Murer, P;Probst, P;Dihr, M;Bajic, D;Neri, D;
Journal
J Gastrointest Oncol