CD82 Antibody Guideline: Preparation, Dilution and Applications

CD82 Antibody Overview

There are 7 CD82 antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA, ELISA(Cap), ELISA(Det), WB, IP. There are 3 CD82 antibody for IHC-P, 4 CD82 antibody for ELISA, 1 CD82 antibody for ELISA(Cap), 1 CD82 antibody for ELISA(Det), 1 CD82 antibody for WB, 1 CD82 antibody for IP. Among all these CD82 antibodies, there are 2 anti-CD82 mouse monoclonal antibodies , 3 anti-CD82 rabbit monoclonal antibodies , 2 anti-CD82 rabbit polyclonal antibodies . All the CD82 anbodies are produced in house and all are in stock. CD82 antibody customerized service is available.

CD82 Antibody ELISA

CD82 antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD82 / KAI-1 (rh CD82 / KAI-1; Catalog#12275-H08H; P27701-1; Gly 111-Leu 228).

CD82 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

CD82 Antibody western blot

CD82 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD82 / KAI-1 (rh CD82 / KAI-1; Catalog#12275-H08H; P27701-1; Gly 111-Leu 228). CD82 / KAI-1 specific IgG was purified by Human CD82 / KAI-1 affinity chromatography.

CD82 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD82 Antibody immunohistochemistry

CD82 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD82 / KAI-1 (rh CD82 / KAI-1; Catalog#12275-H08H; P27701-1; Gly111-Leu228). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD82 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD82 Antibody immunoprecipitation

CD82 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD82 / KAI-1 (rh CD82 / KAI-1; Catalog#12275-H08H; P27701-1; Gly 111-Leu 228). CD82 / KAI-1 specific IgG was purified by Human CD82 / KAI-1 affinity chromatography.

CD82 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes