CD86 Antibody Guideline: Preparation, Dilution and Applications

CD86 Antibody Overview

There are 27 CD86 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ELISA(Cap), ICC/IF, ELISA(Det), IHC-P, WB, IP. There are 16 CD86 antibody for ELISA, 8 CD86 antibody for FCM, 2 CD86 antibody for ELISA(Cap), 1 CD86 antibody for ICC/IF, 2 CD86 antibody for ELISA(Det), 1 CD86 antibody for IHC-P, 1 CD86 antibody for WB, 1 CD86 antibody for IP. Among all these CD86 antibodies, there are 12 anti-CD86 mouse monoclonal antibodies , 7 anti-CD86 rabbit monoclonal antibodies , 8 anti-CD86 rabbit polyclonal antibodies . All the CD86 anbodies are produced in house and all are in stock. CD86 antibody customerized service is available.

CD86 gene family antibody

CD86 Antibody ELISA

CD86 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD86 / B7-2 (rh CD86; Catalog#10699-H08H; NP_008820.2; Met 1-His 239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD86 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD86 Antibody western blot

CD86 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). CD86 specific IgG was purified by mouse CD86 affinity chromatography.

CD86 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD86 Antibody flow cytometry

CD86 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD86 / B7-2 (rR CD86 / B7-2; Catalog#80025-R08H; NP_064466.1; Met1-Ile250). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD86 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD86 Antibody immunohistochemistry

CD86 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD86/B7-2 (rh CD86/B7-2; Catalog#10699-H08H; NP_008820.2; Met1-His239).

CD86 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD86 Antibody immunoprecipitation

CD86 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). CD86 specific IgG was purified by mouse CD86 affinity chromatography.

CD86 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

CD86 antibody citations

Title
Blockade of the Activation of T Cells Around β-Cell by the Targeted CTLA-4 Ig at the Surface of β-Cell
Year
2014
Author
Qu, W;Li, L;Han, C;Li, M;Wang, J;Yin, C;Zhang, J;Jiang, Z;
Journal
Cell Biochem. Biophys.