CEACAM3 Antibody Guideline: Preparation, Dilution and Applications

CEACAM3 Antibody Overview

There are 5 CEACAM3 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, IHC-P. There are 3 CEACAM3 antibody for ELISA, 2 CEACAM3 antibody for FCM, 1 CEACAM3 antibody for IHC-P. Among all these CEACAM3 antibodies, there are 3 anti-CEACAM3 mouse monoclonal antibodies , 2 anti-CEACAM3 rabbit polyclonal antibodies . All the CEACAM3 anbodies are produced in house and all are in stock. CEACAM3 antibody customerized service is available.

CEACAM3 Antibody ELISA

CEACAM3 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM3 / CD66d (rh CEACAM3 / CD66d; Catalog#11933-H08H; NP_001806.2; Met 1-Gly 155). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CEACAM3 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CEACAM3 Antibody flow cytometry

CEACAM3 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM3 / CD66d (rh CEACAM3 / CD66d; Catalog#11933-H08H; NP_001806.2; Met1-Gly155). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CEACAM3 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CEACAM3 Antibody immunohistochemistry

CEACAM3 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CEACAM3/CD66d (rh CEACAM3/CD66d; Catalog#11933-H08H; NP_001806.2; Met1-Gly155). CEACAM3/CD66d specific IgG was purified by Human CEACAM3/CD66d affinity chromatography.

CEACAM3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CEACAM3 antibody citations

Title
CEACAM1 promotes melanoma metastasis and is involved in the regulation of the EMT associated gene network in melanoma cells
Year
2018
Author
Wicklein, D;Otto, B;Suling, A;Elies, E;Lüers, G;Lange, T;Feldhaus, S;Maar, H;Schröder-Schwarz, J;Brunner, G;Wagener, C;Schumacher, U;
Journal
Sci Rep