Chk1 Antibody Guideline: Preparation, Dilution and Applications

Chk1 Antibody Overview

There are 2 Chk1 antibodies which are validated in multiple tissues with various applications, including WB, ICC/IF, ELISA, IP. There are 2 Chk1 antibody for WB, 2 Chk1 antibody for ICC/IF, 1 Chk1 antibody for ELISA, 1 Chk1 antibody for IP. Among all these Chk1 antibodies, there are 2 anti-Chk1 rabbit polyclonal antibodies . All the Chk1 anbodies are produced in house and all are in stock. Chk1 antibody customerized service is available.

Chk1 Antibody ELISA

Chk1 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human Chk1 (rh Chk1; Catalog#10539-H09B; AAM78553.1; Met1-Thr476). Chk1 specific IgG was purified by Human Chk1 affinity chromatography.

Chk1 antibody ELISA validated

Application ELISA
Dilution 1:25000-1:50000
Notes  

Chk1 Antibody western blot

Chk1 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Chk1, and purified by antigen affinity chromatography.

Chk1 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Chk1 Antibody immunoprecipitation

Chk1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human Chk1 (rh Chk1; Catalog#10539-H09B; AAM78553.1; Met1-Thr476). Chk1 specific IgG was purified by Human Chk1 affinity chromatography.

Chk1 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

Chk1 Antibody ICC / IF

Chk1 Antibody ICC / IF Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Chk1, and purified by antigen affinity chromatography.

Chk1 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:1500-1:50000
Notes