CSF1R Antibody Guideline: Preparation, Dilution and Applications

CSF1R Antibody Overview

There are 17 CSF1R antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, IHC-P, WB. There are 15 CSF1R antibody for ELISA, 2 CSF1R antibody for FCM, 3 CSF1R antibody for IHC-P, 1 CSF1R antibody for WB. Among all these CSF1R antibodies, there are 4 anti-CSF1R mouse monoclonal antibodies , 3 anti-CSF1R rabbit monoclonal antibodies , 10 anti-CSF1R rabbit polyclonal antibodies . All the CSF1R anbodies are produced in house and all are in stock. CSF1R antibody customerized service is available.

CSF1R Antibody ELISA

CSF1R antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human M-CSFR extracellular domain (rh M-CSFR; Catalog#10161-H08H; Met 1-Glu 512; NP_005202.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CSF1R antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CSF1R Antibody western blot

CSF1R Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse CSF1R / MCSF Receptor / CD115 (rM CSF1R / MCSF Receptor / CD115; Catalog#50059-M08H; NP_001032948.2; Met 1-Ser 511). Total IgG was purified by Protein A affinity chromatography.

CSF1R Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CSF1R Antibody flow cytometry

CSF1R Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CSF1R / MCSF Receptor / CD115 (rh CSF1R / MCSF Receptor / CD115; Catalog#10161-H08H; NP_005202.2; Met1-Glu512). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CSF1R Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CSF1R Antibody immunohistochemistry

CSF1R Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CSF1R / M-CSFR / CD115 (rh CSF1R / M-CSFR / CD115; Catalog#10161-H20B2; NP_005202.2; Lys543-Asp922). CSF1R / M-CSFR / CD115 specific IgG was purified by Human CSF1R / M-CSFR / CD115 affinity chromatography.

CSF1R Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes