CXCL1 Antibody Guideline: Preparation, Dilution and Applications

CXCL1 Antibody Overview

There are 8 CXCL1 antibodies which are validated in multiple tissues with various applications, including WB, IP, ELISA. There are 3 CXCL1 antibody for WB, 2 CXCL1 antibody for IP, 6 CXCL1 antibody for ELISA. Among all these CXCL1 antibodies, there are 5 anti-CXCL1 rabbit polyclonal antibodies , 2 anti-CXCL1 mouse monoclonal antibodies , 1 anti-CXCL1 rabbit monoclonal antibodies . All the CXCL1 anbodies are produced in house and all are in stock. CXCL1 antibody customerized service is available.

CXCL1 Antibody ELISA

CXCL1 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CXCL1 (rh CXCL1; Catalog#10877-HNCE; P09341-1; Ala35-Asn107). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CXCL1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CXCL1 Antibody western blot

CXCL1 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human CXCL1 , and purified by antigen affinity chromatography.

CXCL1 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CXCL1 Antibody immunoprecipitation

CXCL1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Rat CXCL1 / MGSA / NAP-3 (Catalog#80039-RNCE; NP_110472.1; Ala25-Lys96). CXCL1 / MGSA / NAP-3 specific IgG was purified by Rat CXCL1 / MGSA / NAP-3 affinity chromatography.

CXCL1 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

CXCL1 antibody citations

Title
Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway
Year
2019
Author
Liu, J;Qu, L;Meng, L;Shou, C;
Journal
J. Exp. Clin. Cancer Res.