DLL4 Protein Overview: Sequence, Structure, Function and Protein Interaction

DLL4 Protein Overview

DLL4 reagents

Shutter et al. (2000) identified Dll4 as a mouse EST encoding a polypeptide with homology to the DSL family of Notch ligands. By searching a DNA sequence database with the mouse Dll4 sequence, they identified a partial human brain DLL4 cDNA. Using this partial DLL4 cDNA, Shutter et al. (2000) isolated a full-length human DLL4 coding sequence. The predicted 685-amino acid DLL4 protein exhibits all of the hallmarks of the Delta family of Notch ligands. It has an extracellular region containing 8 EGF-like repeats and a DSL domain involved in Notch binding, as well as a transmembrane domain and a cytoplasmic tail that lacks catalytic motifs. Human DLL4 shares 87% amino acid sequence identity with mouse Dll4. Northern blot analysis of mouse tissues detected highest Dll4 expression in lung, followed by heart, kidney, skeletal muscle, and brain; Dll4 expression was barely detectable in spleen and testis. Northern blot analysis of whole mouse embryos demonstrated increased expression of Dll4 from embryonic day 7 through 17. Analysis by RT-PCR revealed a low level of Dll4 expression in all tissues examined, with higher levels in lung, brown and white adipose, and adrenal. In situ hybridization of mouse tissues showed a strikingly specific expression pattern. In both embryonic and adult tissues, the predominant site of Dll4 expression was the vascular endothelium. Shutter et al. (2000) found a similar expression pattern for human DLL4 by in situ analysis. They suggested that DLL4 is involved in the regulation of vascular biology.

DLL4 protein name

Recommended name
Delta-like protein 4
Alternative name
Drosophila Delta homolog 4 Delta4

DLL4 Protein Sequence

Species Human DLL4 protein
Length 685
Mass (Da) 74605
Sequence Human DLL4 protein sequence
Species Mouse DLL4 protein
Length 686
Mass (Da) 74991
Sequence Mouse DLL4 protein sequence
Species Rat DLL4 protein
Length 686
Mass (Da) 74961
Sequence Rat DLL4 protein sequence

DLL4 Protein Molecular Weight & PI

Delta-like protein 4 precursor (Drosophila Delta homolog 4) (Delta4) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 27-685 Delta-like protein 4.

Molecular weight (Da)


Theoretical pI


DLL4 Protein Structure

Human DLL4 C2-EGF3
2017-01-17   Released:  2017-06-14
Deposition Author(s)
Suckling, R.J., Handford, P.A., Lea, S.M.
Homo sapiens
Expression System
Drosophila melanogaster
Experimental Data Snapshot
2.1700 Å
R-Value Free
R-Value Work

Human DLL4 protein Secondary structure

DLL4 Protein Interaction

Recombinant DLL4 Protein Feature

DLL4 Protein, Human, Recombinant (hFc Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μL/well) can bind biotinylated mouse NOTCH1-his. The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 µg/mL in the presence of 500 ng/mL recombinant human BMP2.

Recombinant DLL4 protein citations

Technical Advance: Ascorbic acid induces development of double-positive T cells from human hematopoietic stem cells in the absence of stromal cells
Huijskens, MJ;Walczak, M;Koller, N;Briedé, JJ;Senden-Gijsbers, BL;Schnijderberg, MC;Bos, GM;Germeraad, WT;
J. Leukoc. Biol.

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