Doublecortin/DCX Antibody Guideline: Preparation, Dilution and Applications

Doublecortin/DCX Antibody Overview

There are 3 Doublecortin/DCX antibodies which are validated in multiple tissues with various applications, including WB, IP, ICC/IF, ELISA. There are 1 Doublecortin/DCX antibody for WB, 1 Doublecortin/DCX antibody for IP, 1 Doublecortin/DCX antibody for ICC/IF, 1 Doublecortin/DCX antibody for ELISA. Among all these Doublecortin/DCX antibodies, there are 2 anti-Doublecortin/DCX rabbit polyclonal antibodies , 1 anti-Doublecortin/DCX mouse monoclonal antibodies . All the Doublecortin/DCX anbodies are produced in house and all are in stock. Doublecortin/DCX antibody customerized service is available.

Doublecortin/DCX gene family antibody

Approved symbol Common name
DCLK1 DCLK1 antibody

Doublecortin/DCX Antibody ELISA

Doublecortin/DCX antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human DCX (rh DCX; Catalog#13498-H09E; O43602-2; Ala45-Val150). Total IgG was purified by Protein A affinity chromatography.

Doublecortin/DCX antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Doublecortin/DCX Antibody western blot

Doublecortin/DCX Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DCX, and purified by antigen affinity chromatography.

Doublecortin/DCX Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Doublecortin/DCX Antibody immunohistochemistry

Doublecortin/DCX Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human DCX (rh DCX; Catalog#13498-H09E; O43602-2; Ala45-Val150). DCX specific IgG was purified by Human DCX affinity chromatography.

Doublecortin/DCX Antibody immunohistochemistry Validated

Application IHC
Dilution 1:2000-1:10000
Notes  

Doublecortin/DCX Antibody immunoprecipitation

Doublecortin/DCX Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DCX, and purified by antigen affinity chromatography.

Doublecortin/DCX Antibody immunoprecipitation Validated

Application IP
Dilution 0.5-2 μL/mg of lysate
Notes  

Doublecortin/DCX Antibody ICC / IF

Doublecortin/DCX Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Doublecortin/DCX (rh Doublecortin/DCX; Catalog#13498-H09E; O43602-2; Ala45-Val150). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Doublecortin/DCX Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes