E-Cadherin Antibody Guideline: Preparation, Dilution and Applications

E-Cadherin Antibody Overview

There are 21 E-Cadherin antibodies which are validated in multiple tissues with various applications, including ELISA(Cap), ICC/IF, ELISA, FCM, IHC-P, WB, IP. There are 1 E-Cadherin antibody for ELISA(Cap), 7 E-Cadherin antibody for ICC/IF, 13 E-Cadherin antibody for ELISA, 4 E-Cadherin antibody for FCM, 5 E-Cadherin antibody for IHC-P, 6 E-Cadherin antibody for WB, 4 E-Cadherin antibody for IP. Among all these E-Cadherin antibodies, there are 8 anti-E-Cadherin mouse monoclonal antibodies , 5 anti-E-Cadherin rabbit monoclonal antibodies , 8 anti-E-Cadherin rabbit polyclonal antibodies . All the E-Cadherin anbodies are produced in house and all are in stock. E-Cadherin antibody customerized service is available.

E-Cadherin Antibody ELISA

E-Cadherin Antibody western blot

E-Cadherin Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human Cadherin-1 / CDH1 (rhCadherin-1; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). Cadherin-1 specific IgG was purified by human Cadherin-1 affinity chromatography.

E-Cadherin Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

E-Cadherin Antibody flow cytometry

E-Cadherin Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human E-Cadherin / CDH1 / E-cad / CD324 (rh E-Cadherin / CDH1 / E-cad / CD324; Catalog#10204-H08H; NP_004351.1; Met1-Ile707). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

E-Cadherin Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

E-Cadherin Antibody immunohistochemistry

E-Cadherin Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Mouse E-Cadherin / CDH1 (rM E-Cadherin / CDH1; Catalog#50671-M08H; P09803; Met1-Val709). Total IgG was purified by Protein A affinity chromatography.

E-Cadherin Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

E-Cadherin Antibody immunoprecipitation

E-Cadherin Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse E-Cadherin / CDH1 (rM E-Cadherin / CDH1; Catalog#50671-M08H; P09803; Met 1-Val 709). E-Cadherin / CDH1 specific IgG was purified by Mouse E-Cadherin / CDH1 affinity chromatography.

E-Cadherin Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

E-Cadherin Antibody ICC / IF

E-Cadherin Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human E-Cadherin / CDH1 / E-cad / CD324 (rh E-Cadherin / CDH1 / E-cad / CD324; Catalog#10204-H08H; NP_004351.1; Met1-Ile707). E-Cadherin / CDH1 / E-cad / CD324 specific IgG was purified by Human E-Cadherin / CDH1 / E-cad / CD324 affinity chromatography.

E-Cadherin Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:50-1:1000
Notes  

E-Cadherin antibody citations

Title
Cellular distribution of the IGF-1R in corneal epithelial cells
Year
2012
Author
Robertson, DM;Zhu, M;Wu, YC;
Journal
Exp. Eye Res.
Title
Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling
Year
2014
Author
Yang, P;Li, Z;Fu, R;Wu, H;Li, Z;
Journal
Cell. Signal.
Title
Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway
Year
2018
Author
Tian, X;Zhou, D;Chen, L;Tian, Y;Zhong, B;Cao, Y;Dong, Q;Zhou, M;Yan, J;Wang, Y;Qiu, Y;Zhang, L;Li, Z;Wang, H;Wang, D;Ying, G;Zhao, Q;
Journal
Cell Death Dis