EGF Antibody Guideline: Preparation, Dilution and Applications

EGF Antibody Overview

There are 12 EGF antibodies which are validated in multiple tissues with various applications, including WB, Neutralization, ELISA, IHC-P. There are 1 EGF antibody for WB, 1 EGF antibody for Neutralization, 10 EGF antibody for ELISA, 1 EGF antibody for IHC-P. Among all these EGF antibodies, there are 7 anti-EGF rabbit polyclonal antibodies , 3 anti-EGF rabbit monoclonal antibodies , 2 anti-EGF mouse monoclonal antibodies . All the EGF anbodies are produced in house and all are in stock. EGF antibody customerized service is available.

EGF Antibody ELISA

EGF antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human EGF(rh EGF; Catalog#10605-HNAE; NP_001954.2; Asn 971-Arg 1023) .

EGF antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000

EGF Antibody western blot

EGF Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Epidermal Growth Factor/EGF, and purified by antigen affinity chromatography.

EGF Antibody western blot Validated

Application WB
Dilution 1:500-1:2000

EGF Antibody immunohistochemistry

EGF Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Rat EGF / Epidermal Growth Factor (Catalog#80446-RNAE; NP_036974.1; Asn974-Arg1026). EGF / Epidermal Growth Factor specific IgG was purified by Rat EGF / Epidermal Growth Factor affinity chromatography.

EGF Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000

EGF Antibody Neutralization

EGF Antibody Neutralization Preparation

EGF Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of EGF Neutralizing Antibody is Measured by its ability to neutralize EGF induced proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. The Neutralization titer (IC50) is typically 2.5-7.5 μg/mL in the presence of 2 ng/mL Recombinant Human EGF.

EGF antibody citations

The antiangiogenic activity of a soluble fragment of the VEGFR extracellular domain
Li, H;Cao, W;Chen, Z;Acheampong, DO;Jin, H;Li, D;Zhang, J;Wang, M;
Biomed. Pharmacother.
Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells
Chen, SW;Himeno, M;Koui, Y;Sugiyama, M;Nishitsuji, H;Mizokami, M;Shimotohno, K;Miyajima, A;Kido, T;
Sci Rep