ENO3 Antibody Guideline: Preparation, Dilution and Applications

ENO3 Antibody Overview

There are 7 ENO3 antibodies which are validated in multiple tissues with various applications, including WB, IP, FCM, ICC/IF, IHC-P, ELISA. There are 5 ENO3 antibody for WB, 3 ENO3 antibody for IP, 1 ENO3 antibody for FCM, 1 ENO3 antibody for ICC/IF, 2 ENO3 antibody for IHC-P, 3 ENO3 antibody for ELISA. Among all these ENO3 antibodies, there are 3 anti-ENO3 rabbit polyclonal antibodies , 2 anti-ENO3 mouse monoclonal antibodies , 2 anti-ENO3 rabbit monoclonal antibodies . All the ENO3 anbodies are produced in house and all are in stock. ENO3 antibody customerized service is available.

ENO3 gene family antibody

Approved symbol Common name
ENO1 ENO1 antibody
Approved symbol Common name
ENO2 NSE/ENO2 antibody

ENO3 Antibody ELISA

ENO3 antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; Catalog#14270-H07E; AAH17249.1; Met1-Lys434).

ENO3 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

ENO3 Antibody western blot

ENO3 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; Catalog#14270-H07E; AAH17249.1; Met1-Lys434). ENO3 / beta-enolase specific IgG was purified by Human ENO3 / beta-enolase affinity chromatography.

ENO3 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

ENO3 Antibody flow cytometry

ENO3 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; Catalog#14270-H07E; AAH17249.1; Met1-Lys434). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ENO3 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

ENO3 Antibody immunohistochemistry

ENO3 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; Catalog#14270-H07E; AAH17249.1; Met1-Lys434).

ENO3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

ENO3 Antibody immunoprecipitation

ENO3 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse ENO3 / beta-enolase, and purified by antigen affinity chromatography.

ENO3 Antibody immunoprecipitation Validated

Application IP
Dilution IP:5-10 μL/mg of lysate
Notes