EpCAM Antibody Guideline: Preparation, Dilution and Applications

EpCAM Antibody Overview

There are 21 EpCAM antibodies which are validated in multiple tissues with various applications, including WB, FCM, ELISA, ELISA(Cap), ELISA(Det), IHC-P, ICC/IF, IP. There are 6 EpCAM antibody for WB, 8 EpCAM antibody for FCM, 11 EpCAM antibody for ELISA, 2 EpCAM antibody for ELISA(Cap), 2 EpCAM antibody for ELISA(Det), 6 EpCAM antibody for IHC-P, 1 EpCAM antibody for ICC/IF, 3 EpCAM antibody for IP. Among all these EpCAM antibodies, there are 6 anti-EpCAM mouse monoclonal antibodies , 7 anti-EpCAM rabbit monoclonal antibodies , 8 anti-EpCAM rabbit polyclonal antibodies . All the EpCAM anbodies are produced in house and all are in stock. EpCAM antibody customerized service is available.

EpCAM Antibody ELISA

EpCAM antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

EpCAM antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

EpCAM Antibody western blot

EpCAM Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EpCAM / TROP-1 / TACSTD1 (rM EpCAM / TROP-1 / TACSTD1; Catalog#50591-M08H; NP_032558.2; Met 1-Thr 266).

EpCAM Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

EpCAM Antibody flow cytometry

EpCAM Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EpCAM / TROP-1 / TACSTD1 (rh EpCAM / TROP-1 / TACSTD1; Catalog#10694-H08H; NP_002345.1; Met1-Lys265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

EpCAM Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

EpCAM Antibody immunohistochemistry

EpCAM Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Mouse EpCAM / TROP-1 / TACSTD1 (rM EpCAM / TROP-1 / TACSTD1; Catalog#50543-M08H; NP_032558.2; Met1-Thr266). EpCAM / TROP-1 / TACSTD1 specific IgG was purified by Mouse Acetylcholinesterase / ACHE affinity chromatography.

EpCAM Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

EpCAM Antibody immunoprecipitation

EpCAM Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Cynomolgus EpCAM / TROP-1 / TACSTD1 (Catalog#90299-CCCH; EHH55554.1; Met1-Lys265). EpCAM / TROP-1 / TACSTD1 specific IgG was purified by Cynomolgus EpCAM / TROP-1 / TACSTD1 affinity chromatography.

EpCAM Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

EpCAM Antibody ICC / IF

EpCAM Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265).

EpCAM Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:250-1:5000
Notes  

EpCAM antibody citations

Title
HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays
Year
2020
Author
Bae, Y;Jang, D;Eom, S;Park, T;Kang, S;
Journal
Sensors and Actuators B: Chemical
Title
HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays
Year
2020
Author
Bae, Y;Jang, D;Eom, S;Park, T;Kang, S;
Journal
Sensors and Actuators B: Chemical
Title
Development of an Improved 3D in vitro Intestinal Model to Perform Permeability Studies of Paracellular Compounds
Year
2020
Author
Macedo, M;Martínez, E;Barrias, C;Sarmento, B;
Journal
Front. Bioeng. Biotechnol.