EphB3 Antibody Guideline: Preparation, Dilution and Applications

EphB3 Antibody Overview

There are 8 EphB3 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, FCM, WB, IP. There are 4 EphB3 antibody for ELISA, 1 EphB3 antibody for IHC-P, 3 EphB3 antibody for FCM, 1 EphB3 antibody for WB, 1 EphB3 antibody for IP. Among all these EphB3 antibodies, there are 4 anti-EphB3 rabbit polyclonal antibodies , 4 anti-EphB3 rabbit monoclonal antibodies . All the EphB3 anbodies are produced in house and all are in stock. EphB3 antibody customerized service is available.

EphB3 Antibody ELISA

EphB3 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human EPHB3 / HEK2 (aa 585-998) (rh EPHB3 / HEK2 (aa 585-998); Catalog#13925-H20B1; P54753; Gln585-Val998). Total IgG was purified by Protein A affinity chromatography.

EphB3 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

EphB3 Antibody western blot

EphB3 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Rat EphB3 / HEK2 / Eph Receptor B3 (rR EphB3 / HEK2 / Eph Receptor B3; Catalog#81593-R08H; NP_001099338.1; Met1-Thr537). EphB3 / HEK2 / Eph Receptor B3 specific IgG was purified by Rat EphB3 / HEK2 / Eph Receptor B3 affinity chromatography.

EphB3 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

EphB3 Antibody flow cytometry

EphB3 Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EphB3 / HEK2 / Eph Receptor B3 (rM EphB3 / HEK2 / Eph Receptor B3; Catalog#50581-M08H; NP_034273.1; Met1-Thr537).

EphB3 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

EphB3 Antibody immunohistochemistry

EphB3 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human EPHB3 / HEK2 (rh EPHB3 / HEK2; Catalog#13925-H20B1; P54753; Gln585-Val998). EPHB3 / HEK2 specific IgG was purified by Human EPHB3 / HEK2 affinity chromatography.

EphB3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

EphB3 Antibody immunoprecipitation

EphB3 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Rat EphB3 / HEK2 / Eph Receptor B3 (rR EphB3 / HEK2 / Eph Receptor B3; Catalog#81593-R08H; NP_001099338.1; Met1-Thr537). EphB3 / HEK2 / Eph Receptor B3 specific IgG was purified by Rat EphB3 / HEK2 / Eph Receptor B3 affinity chromatography.

EphB3 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes