ESAM Antibody Guideline: Preparation, Dilution and Applications

ESAM Antibody Overview

There are 12 ESAM antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA, FCM, WB. There are 1 ESAM antibody for IHC-P, 7 ESAM antibody for ELISA, 4 ESAM antibody for FCM, 1 ESAM antibody for WB. Among all these ESAM antibodies, there are 5 anti-ESAM rabbit polyclonal antibodies , 1 anti-ESAM mouse monoclonal antibodies , 6 anti-ESAM rabbit monoclonal antibodies . All the ESAM anbodies are produced in house and all are in stock. ESAM antibody customerized service is available.

ESAM Antibody ELISA

ESAM antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human extracellular domain (rh ESAM; Catalog#10187-H08H; NP_620411.2; Met 1-Ala 248). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ESAM antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

ESAM Antibody western blot

ESAM Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human ESAM extracellular domain (rh ESAM; Catalog#10187-H08H; NP_620411.2; Met 1-Ala 248). ESAM specific IgG was purified by human ESAM affinity chromatography.

ESAM Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

ESAM Antibody flow cytometry

ESAM Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Endothelial Cell Adhesion Molecule (rh Endothelial Cell Adhesion Molecule; Catalog#10187-H08H; NP_620411.2; Met1-Ala248).

ESAM Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

ESAM Antibody immunohistochemistry

ESAM Antibody immunohistochemistry Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Endothelial Cell Adhesion Molecule, and purified by antigen affinity chromatography.

ESAM Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes