FH/Fumarate Hydratase Antibody Guideline: Preparation, Dilution and Applications

FH/Fumarate Hydratase Antibody Overview

There are 4 FH/Fumarate Hydratase antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP. There are 3 FH/Fumarate Hydratase antibody for WB, 1 FH/Fumarate Hydratase antibody for IHC-P, 3 FH/Fumarate Hydratase antibody for ELISA, 1 FH/Fumarate Hydratase antibody for IP. Among all these FH/Fumarate Hydratase antibodies, there are 3 anti-FH/Fumarate Hydratase rabbit polyclonal antibodies , 1 anti-FH/Fumarate Hydratase mouse monoclonal antibodies . All the FH/Fumarate Hydratase anbodies are produced in house and all are in stock. FH/Fumarate Hydratase antibody customerized service is available.

FH/Fumarate Hydratase Antibody ELISA

FH/Fumarate Hydratase antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human Fumarate Hydratase (rh FH; Catalog#12115-H08E; P07954-1; Ala45-Lys510). Total IgG was purified by Protein A affinity chromatography.

FH/Fumarate Hydratase antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

FH/Fumarate Hydratase Antibody western blot

FH/Fumarate Hydratase Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human Fumarate Hydratase / FH (rh Fumarate Hydratase / FH; Catalog#12115-H08E; P07954-1; Ala 45-Lys 510). Fumarate Hydratase / FH specific IgG was purified by Human Fumarate Hydratase / FH affinity chromatography.

FH/Fumarate Hydratase Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

FH/Fumarate Hydratase Antibody immunohistochemistry

FH/Fumarate Hydratase Antibody immunohistochemistry Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human FH, and purified by antigen affinity chromatography.

FH/Fumarate Hydratase Antibody immunohistochemistry Validated

Application IHC
Dilution 1:200-1:1000
Notes  

FH/Fumarate Hydratase Antibody immunoprecipitation

FH/Fumarate Hydratase Antibody immunoprecipitation Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fumarate Hydratase / FH (rh Fumarate Hydratase / FH; Catalog#12115-H08E; P07954-1; Ala 45-Lys 510). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

FH/Fumarate Hydratase Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes