FLT1 Protein Overview: Sequence, Structure, Function and Protein Interaction

FLT1 Protein Overview

FLT1 reagents

FLT1 protein family

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

FLT1 protein name

Recommended name
Vascular endothelial growth factor receptor 1
Short name
VEGFR-1||FLT-1||FLT
Aliases
vascular endothelial growth factor receptor 1, vascular permeability factor receptor, VEGFR1
Alternative name
Fms-like tyrosine kinase 1 Tyrosine-protein kinase FRT Tyrosine-protein kinase receptor FLT Vascular permeability factor receptor

FLT1 Gene family protein

FLT1 Protein Sequence

Species Human FLT1 protein
Length 1338
Mass (Da) 150769
Sequence Human FLT1 protein sequence
Species Mouse FLT1 protein
Length 1333
Mass (Da) 149876
Sequence Mouse FLT1 protein sequence
Species Rat FLT1 protein
Length 1336
Mass (Da) 150250
Sequence Rat FLT1 protein sequence

FLT1 Protein Molecular Weight & PI

Vascular endothelial growth factor receptor 1 precursor (EC 2.7.10.1) (VEGFR-1) (Fms-like tyrosine kinase 1) (FLT-1) (Tyrosine-protein kinase FRT) (Tyrosine-protein kinase receptor FLT) (FLT) (Vascular permeability factor receptor) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 27-1338 Vascular endothelial growth factor

Molecular weight (Da)

148096.48

Theoretical pI

8.72

FLT1 Protein Structure

Crystal structure of VEGFR-1 domain 2 in presence of Cobalt
Deposited
2014-01-13   Released:  2015-01-28
Deposition Author(s)
Gaucher, J.-F., Reille-Seroussi, M., Gagey-Eilstein, N., Broussy, S., Coric, P., Seijo, B., Lascombe, M.-B., Gautier, B., Liu, W.-Q., Huguenot, F., Inguimbert, N., Bouaziz, S., Vidal, M., Broutin, I.
Organism(s)
Homo sapiens
Expression System
Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.0000 Å
R-Value Free
0.233
R-Value Work
0.168
4CL7 From PDB

Human FLT1 protein Secondary structure

FLT1 Protein Interaction

Recombinant FLT1 Protein Feature

FLT1 Protein, Human, Recombinant (His Tag)

High Purity
> 97 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al.,1990 , Proc. Natl. Acad. Sci. USA 87:1323.). The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.

Recombinant FLT1 protein citations

Title
Serum-dependence of affinity-mediated VEGF release from biomimetic microspheres
Year
2014
Author
DG Belair, AS Khalil, MJ Miller, WL Murphy
Journal
Biomacromolecules

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