Glypican 3 Antibody Guideline: Preparation, Dilution and Applications

Glypican 3 Antibody Overview

There are 7 Glypican 3 antibodies which are validated in multiple tissues with various applications, including FCM, ICC/IF, WB, ELISA. There are 4 Glypican 3 antibody for FCM, 2 Glypican 3 antibody for ICC/IF, 1 Glypican 3 antibody for WB, 2 Glypican 3 antibody for ELISA. Among all these Glypican 3 antibodies, there are 5 anti-Glypican 3 rabbit monoclonal antibodies , 2 anti-Glypican 3 rabbit polyclonal antibodies . All the Glypican 3 anbodies are produced in house and all are in stock. Glypican 3 antibody customerized service is available.

Glypican 3 gene family antibody

Approved symbol Common name
GPC5 Glypican 5 antibody

Glypican 3 Antibody ELISA

Glypican 3 antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Glypican 3/GPC3/OCI-5 (rM Glypican 3/GPC3/OCI-5; Catalog#50989-M08B; NP_057906.2; Met1-Pro558).

Glypican 3 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

Glypican 3 Antibody western blot

Glypican 3 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Glypican 3 / GPC3 / OCI-5. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Glypican 3 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Glypican 3 Antibody flow cytometry

Glypican 3 Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Glypican 3 / GPC3 / OCI-5. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Glypican 3 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

Glypican 3 Antibody immunohistochemistry

Glypican 3 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Glypican 3 / GPC3 / OCI-5. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Glypican 3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:20-1:80
Notes  

Glypican 3 Antibody immunoprecipitation

Glypican 3 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Glypican 3 / GPC3 / OCI-5, and purified by antigen affinity chromatography.

Glypican 3 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes