GOLPH2/GOLM1 Antibody Guideline: Preparation, Dilution and Applications

GOLPH2/GOLM1 Antibody Overview

There are 21 GOLPH2/GOLM1 antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA, ICC/IF, FCM, WB, IP. There are 7 GOLPH2/GOLM1 antibody for IHC-P, 10 GOLPH2/GOLM1 antibody for ELISA, 5 GOLPH2/GOLM1 antibody for ICC/IF, 6 GOLPH2/GOLM1 antibody for FCM, 4 GOLPH2/GOLM1 antibody for WB, 3 GOLPH2/GOLM1 antibody for IP. Among all these GOLPH2/GOLM1 antibodies, there are 6 anti-GOLPH2/GOLM1 mouse monoclonal antibodies , 8 anti-GOLPH2/GOLM1 rabbit monoclonal antibodies , 7 anti-GOLPH2/GOLM1 rabbit polyclonal antibodies . All the GOLPH2/GOLM1 anbodies are produced in house and all are in stock. GOLPH2/GOLM1 antibody customerized service is available.

GOLPH2/GOLM1 Antibody ELISA

GOLPH2/GOLM1 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H07E; Q8NBJ4-1; Met1-Leu401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

GOLPH2/GOLM1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

GOLPH2/GOLM1 Antibody western blot

GOLPH2/GOLM1 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H07E; Q8NBJ4-1; Met1-Leu401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

GOLPH2/GOLM1 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

GOLPH2/GOLM1 Antibody flow cytometry

GOLPH2/GOLM1 Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human GOLM1 (rh GOLM1; Catalog#13066-H08H; Q8NBJ4-1; Val 40-Leu 401).

GOLPH2/GOLM1 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

GOLPH2/GOLM1 Antibody immunohistochemistry

GOLPH2/GOLM1 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H08H; Q8NBJ4-1; Val 40-Leu 401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

GOLPH2/GOLM1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

GOLPH2/GOLM1 Antibody immunoprecipitation

GOLPH2/GOLM1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73; Catalog#13066-H07E; Q8NBJ4-1; Met 1-Leu 401). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.

GOLPH2/GOLM1 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

GOLPH2/GOLM1 Antibody ICC / IF

GOLPH2/GOLM1 antibody citations

Title
A Novel Electrochemical Immunosensor for Golgi Protein 73 Assay
Year
2014
Author
Yu, Y;Gao, T;Li, H;Ye, Z;Chen, Z;Li, G;
Journal
Electrochemistry Communications
Title
A Novel Electrochemical Immunosensor for Golgi Protein 73 Assay
Year
2014
Author
Yu, Y;Gao, T;Li, H;Ye, Z;Chen, Z;Li, G;
Journal
Electrochemistry Communications
Title
Ratiometric Immunosensor for GP73 Detection Based on the Ratios of Electrochemiluminescence and Electrochemical Signal Using DNA Tetrahedral Nanostructure as the Carrier of Stable Reference Signal
Year
2019
Author
Lin, Y;Jia, J;Yang, R;Chen, D;Wang, J;Luo, F;Guo, L;Qiu, B;Lin, Z;
Journal
Anal. Chem.