Granzyme B Antibody Guideline: Preparation, Dilution and Applications

Granzyme B Antibody Overview

There are 5 Granzyme B antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, WB. There are 5 Granzyme B antibody for ELISA, 3 Granzyme B antibody for IHC-P, 1 Granzyme B antibody for WB. Among all these Granzyme B antibodies, there are 1 anti-Granzyme B mouse monoclonal antibodies , 1 anti-Granzyme B rabbit monoclonal antibodies , 3 anti-Granzyme B rabbit polyclonal antibodies . All the Granzyme B anbodies are produced in house and all are in stock. Granzyme B antibody customerized service is available.

Granzyme B Antibody ELISA

Granzyme B antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human Granzyme B (rhGranzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). Total IgG was purified by Protein A affinity chromatography.

Granzyme B antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Granzyme B Antibody western blot

Granzyme B Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human Granzyme B/GZMB (rh Granzyme B/GZMB; Catalog#10345-H08H; NP_004122.1; Met1-Tyr247). Granzyme B/GZMB specific IgG was purified by Human Granzyme B/GZMB affinity chromatography.

Granzyme B Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Granzyme B Antibody immunohistochemistry

Granzyme B Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme B (rhGranzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Granzyme B Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

Granzyme B Antibody immunoprecipitation

Granzyme B Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human Granzyme B (rh Granzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). Granzyme B specific IgG was purified by human Granzyme B affinity chromatography.

Granzyme B Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes