HA tag Antibody Guideline: Preparation, Dilution and Applications

HA tag Antibody Overview

There are 3 HA tag antibodies which are validated in multiple tissues with various applications, including WB, ELISA, FCM, ICC/IF, IP. There are 1 HA tag antibody for WB, 2 HA tag antibody for ELISA, 1 HA tag antibody for FCM, 1 HA tag antibody for ICC/IF, 2 HA tag antibody for IP. Among all these HA tag antibodies, there are 3 anti-HA tag mouse monoclonal antibodies . All the HA tag anbodies are produced in house and all are in stock. HA tag antibody customerized service is available.

HA tag Antibody ELISA

HA tag antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HA tag antibody ELISA validated

Application ELISA
Dilution 1/2000-1/5000
Notes  

HA tag Antibody western blot

HA tag Antibody western blot Preparation

Produced in rabbits immunized with a purified synthetic peptide corresponding to the HA-tag sequence. HA-tag specific IgG was purified by HA-tag affinity chromatography.

HA tag Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

HA tag Antibody flow cytometry

HA tag Antibody immunoprecipitation

HA tag Antibody immunoprecipitation Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HA tag Antibody immunoprecipitation Validated

Application IP
Dilution 4-8 μL/mg of lysate
Notes  

HA tag Antibody ICC / IF

HA tag Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HA tag Antibody ICC / IF Validated

Application ICC_IF
Dilution 1/200-1/500
Notes  

HA tag antibody citations

Title
Infection-derived lipids elicit an immune deficiency circuit in arthropods
Year
2017
Author
Shaw, DK;Wang, X;Brown, LJ;Chávez, AS;Reif, KE;Smith, AA;Scott, AJ;McClure, EE;Boradia, VM;Hammond, HL;Sundberg, EJ;Snyder, GA;Liu, L;DePonte, K;Villar, M;Ueti, MW;de la Fuente, J;Ernst, RK;Pal, U;Fikrig, E;Pedra, JH;
Journal
Nat Commun