HGF Antibody Guideline: Preparation, Dilution and Applications

HGF Antibody Overview

There are 19 HGF antibodies which are validated in multiple tissues with various applications, including Neutralization, ELISA, IHC-P, ICC/IF, WB. There are 1 HGF antibody for Neutralization, 17 HGF antibody for ELISA, 7 HGF antibody for IHC-P, 1 HGF antibody for ICC/IF, 1 HGF antibody for WB. Among all these HGF antibodies, there are 1 anti-HGF chimera monoclonal antibodies , 1 anti-HGF mouse monoclonal antibodies , 5 anti-HGF rabbit monoclonal antibodies , 12 anti-HGF rabbit polyclonal antibodies . All the HGF anbodies are produced in house and all are in stock. HGF antibody customerized service is available.

HGF gene family antibody

Approved symbol Common name
CIB2 CIB2 antibody
DIABLO SMAC antibody
MET c-MET antibody
SerpinB6 SerpinB6 antibody

HGF Antibody ELISA

HGF antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HGF / Hepatocyte Growth Factor (rh HGF / Hepatocyte Growth Factor; Catalog#10463-H08H; NP_000592.3; Met 1-Ser 728). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HGF antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

HGF Antibody western blot

HGF Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Rat HGF/Hepatocyte Growth Factor (rR HGF/Hepatocyte Growth Factor; Catalog#80429-RNAH; NP_058713.1; Met1-Leu728). HGF/Hepatocyte Growth Factor specific IgG was purified by Rat HGF/Hepatocyte Growth Factor affinity chromatography.

HGF Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

HGF Antibody immunohistochemistry

HGF Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human HGF (rh HGF; Catalog#10463-HNAS; NP_000592.3; Met 1-Ser 728). HGF specific IgG was purified by Human HGF affinity chromatography.

HGF Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

HGF Antibody Neutralization

HGF Antibody Neutralization Preparation

HGF Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of HGF Neutralizing Antibody is Measured by its ability to neutralize autocrine HGF-induced proliferation in the U87 MG cell line. Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Monoclonal Antibody. The IC50 is typically 1-4 μg/mL.
Notes  

HGF antibody citations

Title
Analysis of soluble factors in conditioned media derived from primary cultures of cirrhotic liver of biliary atresia
Year
2017
Author
Yamazaki, T;Wakai, M;Enosawa, S;Tokiwa, T;
Journal
In Vitro Cell. Dev. Biol. Anim
Title
Impaired integrin α5 /β1 -mediated hepatocyte growth factor release by stellate cells of the aged liver
Year
2020
Author
Rohn, F;Kordes, C;Buschmann, T;Reichert, D;Wammers, M;Poschmann, G;Stühler, K;Benk, AS;Geiger, F;Spatz, JP;Häussinger, D;
Journal
Aging Cell
Title
The role of IL-6 trans-signaling in liver regeneration after partial hepatectomy Dissertation
Year
2020
Author
Modares, NF;
Journal
Thesis