HIF-1 alpha Antibody Guideline: Preparation, Dilution and Applications

HIF-1 alpha Antibody Overview

There are 5 HIF-1 alpha antibodies which are validated in multiple tissues with various applications, including IHC-P, ICC/IF, ELISA. There are 1 HIF-1 alpha antibody for IHC-P, 2 HIF-1 alpha antibody for ICC/IF, 2 HIF-1 alpha antibody for ELISA. Among all these HIF-1 alpha antibodies, there are 1 anti-HIF-1 alpha mouse monoclonal antibodies , 1 anti-HIF-1 alpha rabbit monoclonal antibodies , 3 anti-HIF-1 alpha rabbit polyclonal antibodies . All the HIF-1 alpha anbodies are produced in house and all are in stock. HIF-1 alpha antibody customerized service is available.

HIF-1 alpha gene family antibody

Approved symbol Common name
MAX MAX antibody
Approved symbol Common name
MITF MITF antibody

HIF-1 alpha Antibody ELISA

HIF-1 alpha antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human HIF1A (rh HIF1A; Catalog#11977-H07E; Q16665-1; Arg 575-Asn 826). Total IgG was purified by Protein A affinity chromatography .

HIF-1 alpha antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

HIF-1 alpha Antibody immunohistochemistry

HIF-1 alpha Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HIF-1 alpha / HIF1A (rh HIF-1 alpha / HIF1A; Catalog#11977-H07E; Q16665-1; Arg575-Asn826). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HIF-1 alpha Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

HIF-1 alpha Antibody ICC / IF

HIF-1 alpha Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human HIF-1 alpha / HIF1A (rh HIF-1 alpha / HIF1A; Catalog#11977-H07E; Q16665-1; Arg575-Asn826).

HIF-1 alpha Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes