Histone H3 Antibody Guideline: Preparation, Dilution and Applications

Histone H3 Antibody Overview

There are 2 Histone H3 antibodies which are validated in multiple tissues with various applications, including ICC/IF, WB, IHC-P. There are 1 Histone H3 antibody for ICC/IF, 1 Histone H3 antibody for WB, 1 Histone H3 antibody for IHC-P. Among all these Histone H3 antibodies, there are 2 anti-Histone H3 mouse monoclonal antibodies . All the Histone H3 anbodies are produced in house and all are in stock. Histone H3 antibody customerized service is available.

Histone H3 Antibody western blot

Histone H3 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Histone H3 Antibody western blot Validated

Application WB
Dilution 1/1000-1/100000
Notes  

Histone H3 Antibody immunohistochemistry

Histone H3 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Histone H3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1/50-1/1000
Notes  

Histone H3 Antibody ICC / IF

Histone H3 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Histone H3 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

Histone H3 antibody citations

Title
Triazole-dithiocarbamate based selective lysine specific demethylase 1 (LSD1) inactivators inhibit gastric cancer cell growth, invasion, and migration
Year
2013
Author
Zheng, YC;Duan, YC;Ma, JL;Xu, RM;Zi, X;Lv, WL;Wang, MM;Ye, XW;Zhu, S;Mobley, D;Zhu, YY;Wang, JW;Li, JF;Wang, ZR;Zhao, W;Liu, HM;
Journal
J. Med. Chem.
Title
Design, Synthesis, and Structure-Activity Relationship of Novel LSD1 Inhibitors Based on Pyrimidine-Thiourea Hybrids As Potent, Orally Active Antitumor Agents
Year
2015
Author
Ma, LY;Zheng, YC;Wang, SQ;Wang, B;Wang, ZR;Pang, LP;Zhang, M;Wang, JW;Ding, L;Li, J;Wang, C;Hu, B;Liu, Y;Zhang, XD;Wang, JJ;Wang, ZJ;Zhao, W;Liu, HM;
Journal
J. Med. Chem.
Title
Point mutation impairs centromeric CENH3 loading and induces haploid plants
Year
2015
Author
Karimi-Ashtiyani, R;Ishii, T;Niessen, M;Stein, N;Heckmann, S;Gurushidze, M;Banaei-Moghaddam, AM;Fuchs, J;Schubert, V;Koch, K;Weiss, O;Demidov, D;Schmidt, K;Kumlehn, J;Houben, A;
Journal
Proc. Natl. Acad. Sci. U.S.A.
Title
The Tet1 and histone methylation expression pattern in dairy goat testis
Year
2015
Author
Zheng, L;Zhu, H;Tang, F;Mu, H;Li, N;Wu, J;Hua, J;
Journal
Theriogenology
Title
The distribution of α-kleisin during meiosis in the holocentromeric plant Luzula elegans
Year
2016
Author
Ma, W;Schubert, V;Martis, MM;Hause, G;Liu, Z;Shen, Y;Conrad, U;Shi, W;Scholz, U;Taudien, S;Cheng, Z;Houben, A;
Journal
Chromosome Res.