HIV gp120 Antibody Guideline: Preparation, Dilution and Applications

HIV gp120 Antibody Overview

There are 7 HIV gp120 antibodies which are validated in multiple tissues with various applications, including WB, ELISA, ELISA(Cap), ELISA(Det). There are 5 HIV gp120 antibody for WB, 7 HIV gp120 antibody for ELISA, 1 HIV gp120 antibody for ELISA(Cap), 1 HIV gp120 antibody for ELISA(Det). Among all these HIV gp120 antibodies, there are 1 anti-HIV gp120 mouse monoclonal antibodies , 3 anti-HIV gp120 rabbit monoclonal antibodies , 3 anti-HIV gp120 rabbit polyclonal antibodies . All the HIV gp120 anbodies are produced in house and all are in stock. HIV gp120 antibody customerized service is available.

HIV gp120 Antibody ELISA

HIV gp120 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HIV-1 gp120 (Catalog#11233-V08H; Ala 29-Glu 503; AFM77980.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HIV gp120 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

HIV gp120 Antibody western blot

HIV gp120 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant HIV-1 gp120 (rv GP120; Catalog#11233-V08H; Ala 29-Glu 503; AFM77980.1).

HIV gp120 Antibody western blot Validated

Application WB
Dilution his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect HIV-1 gp120 in WB. Using a DAB detection system, the detection limit for HIV-1 gp120 is approximately 0.25 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
Notes  

HIV gp120 Antibody flow cytometry

HIV gp120 Antibody immunohistochemistry

HIV gp120 Antibody immunoprecipitation

HIV gp120 Antibody ICC / IF

HIV gp120 antibody citations

Title
Three-Dimensional Imaging of HIV-1 Virological Synapses Reveals Membrane Architectures Involved in Virus Transmission
Year
2014
Author
T Do, G Murphy, LA Earl, GQ Del Prete…
Journal
Journal of Virology
Title
Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization
Year
2017
Author
Ouyang, W;Han, J;Wang, W;
Journal
Lab Chip
Title
Trilobatin as an HIV-1 Entry Inhibitor Targeting the HIV-1 Gp41 Envelope
Year
2018
Author
Yin, S;Zhang, X;Lai, F;Liang, T;Wen, J;Lin, W;Qiu, J;Liu, S;Li, L;
Journal
FEBS Lett.