HMGB1 Antibody Guideline: Preparation, Dilution and Applications

HMGB1 Antibody Overview

There are 2 HMGB1 antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ICC/IF, IP. There are 1 HMGB1 antibody for WB, 1 HMGB1 antibody for IHC-P, 2 HMGB1 antibody for ICC/IF, 1 HMGB1 antibody for IP. Among all these HMGB1 antibodies, there are 1 anti-HMGB1 rabbit polyclonal antibodies , 1 anti-HMGB1 mouse monoclonal antibodies . All the HMGB1 anbodies are produced in house and all are in stock. HMGB1 antibody customerized service is available.

HMGB1 gene family antibody

Approved symbol Common name
HMGN3 HMGN3 antibody

HMGB1 Antibody western blot

HMGB1 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HMGB1/HMG1 , and purified by antigen affinity chromatography.

HMGB1 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

HMGB1 Antibody immunohistochemistry

HMGB1 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HMGB1/HMG1 , and purified by antigen affinity chromatography.

HMGB1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

HMGB1 Antibody immunoprecipitation

HMGB1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HMGB1/HMG1 , and purified by antigen affinity chromatography.

HMGB1 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

HMGB1 Antibody ICC / IF

HMGB1 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HMGB1 / HMG1 (rh HMGB1 / HMG1 ; Catalog#10326-H08H; NP_002119.1; Met1-Glu215). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HMGB1 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

HMGB1 antibody citations

Title
Ulinastatin Preconditioning Attenuates Inflammatory Reaction of Hepatic Ischemia Reperfusion Injury in Rats via High Mobility Group Box 1 (HMGB1) …
Year
2014
Author
Y Tong, Z Tang, T Yang, Y Yang, L Yang…
Journal
International Journal of Medic al Sc iences