HMGB1 Protein Overview: Sequence, Structure, Function and Protein Interaction

HMGB1 Protein Overview

The HMG (high mobility group) proteins are nonhistone chromosomal proteins. Wen et al. (1989) isolated a cDNA clone that encodes the entire 215-amino acid sequence of HMG1 from a human placenta cDNA library. Northern blot analysis showed that 3 mRNA species of approximately 1.0, 1.4, and 2.4 kb were expressed in all mammalian organs and cell lines examined. The HMG1 protein has a molecular mass of approximately 25 kD.

HMGB1 protein family

Belongs to the HMGB family.

HMGB1 protein name

Recommended name
High mobility group protein B1
Short name
HMG-1
Aliases
Amphoterin, DKFZp686A04236, high mobility group box 1, high mobility group protein 1, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein
Alternative name
High mobility group protein 1

HMGB1 Gene family protein

Canonical high mobility group
Approved symbol Common name
HMGN3 HMGN3 protein, recombinant

HMGB1 Protein Sequence

Species Human HMGB1 protein
Length 215
Mass (Da) 24894
Sequence Human HMGB1 protein sequence
Species Mouse HMGB1 protein
Length 215
Mass (Da) 24894
Sequence Mouse HMGB1 protein sequence
Species Rat HMGB1 protein
Length 215
Mass (Da) 24894
Sequence Rat HMGB1 protein sequence

HMGB1 Protein Molecular Weight & PI

High mobility group protein B1 (High mobility group protein 1) (HMG-1) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-215 High mobility group protein B1.

Molecular weight (Da)

24893.76

Theoretical pI

5.60

HMGB1 Protein Structure

Pre-Reaction Complex, RAG1(E962Q)/2-intact/nicked 12/23RSS complex in Mn2+
Deposited
2018-02-24   Released:  2018-04-25
Deposition Author(s)
Chuenchor, W., Chen, X., Kim, M.S., Gellert, M., Yang, W.
Organism(s)
Mus musculus, Homo sapiens
Expression System
Homo sapiens, Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.1500 Å
R-Value Free
0.249
R-Value Work
0.209
6CIK From PDB

Human HMGB1 protein Secondary structure

HMGB1 Protein Interaction

Recombinant HMGB1 Protein Feature

HMGB1 Protein, Human, Recombinant (hFc Tag)

High Purity
> 97 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse AGER at 2 μg/ml (100 μl/well) can bind human HMGB1. The EC50 of human HMGB1 is 0.23 μg/ml .

Recombinant HMGB1 protein citations

Title
Effects of high-mobility group box 1 on the proliferation and odontoblastic differentiation of human dental pulp cells
Year
2013
Author
Qi, SC;Cui, C;Yan, YH;Sun, GH;Zhu, SR;
Journal
Int Endod J
Title
The protection of 4,4'-diphenylmethane-bis(methyl) carbamate from Cortex Mori on advanced glycation end product-induced endothelial dysfunction: via inhibiting AGE formation or blocking AGEs-RAGE axis?
Year
2013
Author
Feng, L;Zhu, M;Zhang, M;Gu, J;Jia, X;Tan, X;Gao, C;Zhu, Q;
Journal
Fitoterapia
Title
The protection of 4,4'-diphenylmethane-bis(methyl) carbamate from Cortex Mori on advanced glycation end product-induced endothelial dysfunction: via inhibiting AGE formation or blocking AGEs-RAGE axis?
Year
2013
Author
Feng, L;Zhu, M;Zhang, M;Gu, J;Jia, X;Tan, X;Gao, C;Zhu, Q;
Journal
Fitoterapia
Title
Suppression of toll-like receptor 2 expression inhibits the bioactivity of human hepatocellular carcinoma
Year
2014
Author
Shi, W;Su, L;Li, Q;Sun, L;Lv, J;Li, J;Cheng, B;
Journal
Tumour Biol.
Title
High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma
Year
2015
Author
Xu, YF;Ge, FJ;Han, B;Yang, XQ;Su, H;Zhao, AC;Zhao, MH;Yang, YB;Yang, J;
Journal
World J. Gastroenterol.
Title
Involvement of high mobility group box-1 in imiquimod-induced psoriasis-like mice model
Year
2016
Author
Chen, T;Fu, LX;Guo, ZP;Yin, B;Cao, N;Qin, S;
Journal
J. Dermatol
Title
Regulation of Autophagy-Related Protein and Cell Differentiation by High Mobility Group Box 1 Protein in Adipocytes
Year
2016
Author
Feng, H;Yu, L;Zhang, G;Liu, G;Yang, C;Wang, H;Song, X;
Journal
Mediators Inflamm.
Title
High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen‑activated protein kinase signaling pathway
Year
2017
Author
Zhou, Y;Han, Q;Wang, L;Liu, T;Meng, X;Wu, L;Li, T;Jiao, Y;Yao, H;Zhang, D;
Journal
Experimental and Therapeutic Medicine
Title
HMGB1-induced autophagy: a new pathway to maintain Treg function during chronic hepatitis B virus infection
Year
2017
Author
Cheng, LS;Li, J;Liu, Y;Wang, FP;Wang, SQ;She, WM;Wu, SD;Qi, XL;Zhou, YP;Jiang, W;
Journal
Clin. Sci
Title
High-Mobility Group Box 1 From Hypoxic Trophoblasts Promotes Endothelial Microparticle Production and Thrombophilia in Preeclampsia
Year
2018
Author
Hu, Y;Yan, R;Zhang, C;Zhou, Z;Liu, M;Wang, C;Zhang, H;Dong, L;Zhou, T;Wu, Y;Dong, N;Wu, Q;
Journal
Arterioscler. Thromb. Vasc. Biol.
Title
HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis
Year
2018
Author
Li, J;Zeng, C;Zheng, B;Liu, C;Tang, M;Jiang, Y;Chang, Y;Song, W;Wang, Y;Yang, C;
Journal
Clin. Sci.
Title
HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis
Year
2018
Author
Li, J;Zeng, C;Zheng, B;Liu, C;Tang, M;Jiang, Y;Chang, Y;Song, W;Wang, Y;Yang, C;
Journal
Clin. Sci.
Title
Arctigenin protects against depression by inhibiting microglial activation and neuroinflammation via HMGB1/TLR4/NF‐κB and TNF‐α/TNFR1/NF‐κB pathways
Year
2020
Author
Xu, X;Piao, HN;Aosai, F;Zeng, XY;Cheng, JH;
Journal
British Journal of Pharmacology

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