ICAM-1 Antibody Guideline: Preparation, Dilution and Applications

ICAM-1 Antibody Overview

There are 31 ICAM-1 antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Cap), FCM, ICC/IF, IHC-P, WB, IP, ELISA(Det). There are 15 ICAM-1 antibody for ELISA, 3 ICAM-1 antibody for ELISA(Cap), 12 ICAM-1 antibody for FCM, 5 ICAM-1 antibody for ICC/IF, 10 ICAM-1 antibody for IHC-P, 2 ICAM-1 antibody for WB, 3 ICAM-1 antibody for IP, 2 ICAM-1 antibody for ELISA(Det). Among all these ICAM-1 antibodies, there are 8 anti-ICAM-1 mouse monoclonal antibodies , 14 anti-ICAM-1 rabbit monoclonal antibodies , 9 anti-ICAM-1 rabbit polyclonal antibodies . All the ICAM-1 anbodies are produced in house and all are in stock. ICAM-1 antibody customerized service is available.

ICAM-1 gene family antibody

ICAM-1 Antibody ELISA

ICAM-1 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 extracellular domain (rh ICAM1; Catalog#10346-H08H; NP_000192.2; Met 1-Glu 480). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ICAM-1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

ICAM-1 Antibody western blot

ICAM-1 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human ICAM1 / CD54 extracellular domain (rh ICAM1; Catalog#10346-H08H; NP_000192.2; Met 1-Glu 480). ICAM1 specific IgG was purified by human ICAM1 affinity chromatography.

ICAM-1 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

ICAM-1 Antibody flow cytometry

ICAM-1 Antibody immunohistochemistry

ICAM-1 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD54 / ICAM1 (rM CD54 / ICAM1; Catalog#50440-M08H; NP_034623.1; Met 1-Asn 485).

ICAM-1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

ICAM-1 Antibody immunoprecipitation

ICAM-1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Rat ICAM-1 / CD54 (rR ICAM-1 / CD54; Catalog#80022-R08H; Q00238-1; Met 1-Thr 493). ICAM-1 / CD54 specific IgG was purified by Rat ICAM-1 / CD54 affinity chromatography.

ICAM-1 Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

ICAM-1 Antibody ICC / IF

ICAM-1 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 (rh ICAM1 / CD54; Catalog#10346-H08H; Met 1-Glu 480; NP_000192.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. ved.

ICAM-1 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

ICAM-1 antibody citations

Title
A non-invasive nanoparticle mediated delivery of triamcinolone acetonide ameliorates diabetic retinopathy in rats
Year
2018
Author
Mahaling, B;Srinivasarao, D;Raghu, G;Kasam, R;Reddy, G;Katti, D;
Journal
Nanoscale
Title
Distinct Pathogenesis of Pancreatic Cancer Microvesicle-Associated Venous Thrombosis Identifies New Antithrombotic Targets In Vivo
Year
2018
Author
Stark, K;Schubert, I;Joshi, U;Kilani, B;Hoseinpour, P;Thakur, M;Grünauer, P;Pfeiler, S;Schmidergall, T;Stockhausen, S;Bäumer, M;Chandraratne, S;von Brühl, ML;Lorenz, M;Coletti, R;Reese, S;Laitinen, I;Wörmann, SM;Algül, H;Bruns, CJ;Ware, J;Mackman, N;Engelmann, B;Massberg, S;
Journal
Arterioscler. Thromb. Vasc. Biol.