IgG Antibody Guideline: Preparation, Dilution and Applications

IgG Antibody Overview

There are 2 IgG antibodies which are validated in multiple tissues with various applications, including ELISA. There are 2 IgG antibody for ELISA. Among all these IgG antibodies, there are 2 anti-IgG rabbit polyclonal antibodies . All the IgG anbodies are produced in house and all are in stock. IgG antibody customerized service is available.

IgG Antibody ELISA

IgG antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant human IgG1-Fc (Catalog#10702-HNAH; P01857-1; Glu99-Lys330,C103S). This antibody was purified by Protein A affinity chromatography.

IgG antibody ELISA validated

Application ELISA
Dilution 1:5000-1:20000
Notes  

IgG antibody citations

Title
Reconstruction of H3N2 influenza virus based virosome in-vitro
Year
2013
Author
A Abdoli, H Soleimanjahi, MT Kheiri…
Journal
Iranian journal of microbiology.
Title
A crucial role of anti‐RPLP0 and anti‐galectin‐3 antibodies in the development of skin lesions in systemic lupus erythematosus
Year
2014
Author
Z Shi, G Tan, Z Meng, M Yu, K Li, J Yin…
Journal
Arthritis & Rheumatology
Title
Disparate Regulatory Mechanisms Control Fat3 and P75 NTR Protein Transport through a Conserved Kif5-Interaction Domain
Year
2016
Author
Cheng, H;Garcia, JB;Birkness, JE;Trinidad, JC;Deans, MR;
Journal
PloS one
Title
Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer
Year
2016
Author
Zhang, J;Jiang, Z;Liu, X;Meng, A;
Journal
Development
Title
Synergistic antitumor effect of a Human Papillomavirus DNA vaccine harboring E6E7 fusion gene and vascular endothelial growth factor receptor 2 gene
Year
2016
Author
Gao, J;Fan, L;Ma, W;Xiao, H;
Journal
Microbiol. Immunol.
Title
A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies
Year
2017
Author
Pleiner, T;Bates, M;Görlich, D;
Journal
J. Cell Biol.
Title
A polymyxin B–silver nanoparticle colloidal system and the application of lipopolysaccharide analysis
Year
2018
Author
Liu, T;Gao, L;Zhao, J;Cao, Y;Tang, Y;Miao, P;
Journal
Analyst
Title
High COX-2 expression contributes to a poor prognosis through the inhibition of chemotherapy-induced senescence in nasopharyngeal carcinoma
Year
2018
Author
Shi, C;Guan, Y;Zeng, L;Liu, G;Zhu, Y;Xu, H;Lu, Y;Liu, J;Guo, J;Feng, X;Zhao, X;Jiang, W;Li, G;Li, G;Dai, Y;Jin, F;Li, W;Zhou, W;
Journal
Int. J. Oncol.
Title
Tumor associated macrophages deliver iron to tumor cells via Lcn2
Year
2018
Author
Duan, X;He, K;Li, J;Cheng, M;Song, H;Liu, J;Liu, P;
Journal
Int J Physiol Pathophysiol Pharmacol
Title
Automatic Spot Identification Method for High Throughput Surface Plasmon Resonance Imaging Analysis
Year
2018
Author
Wang, Z;Huang, X;Cheng, Z;
Journal
Biosensors (Basel)
Title
Detection of SARS-CoV-2-specific humoral and cellular immunity in COVID-19 convalescent individuals
Year
2020
Author
Ni, L;Ye, F;Cheng, M;Feng, Y;Deng, Y;Zhao, H;Wei, P;Ge, J;Gou, M;Li, X;Sun, L;Cao, T;Wang, P;Zhou, C;Zhang, R;Liang, P;Guo, H;Wang, X;Qin, C;Chen, F;Dong, C;
Journal
Immunity
Title
The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells
Year
2020
Author
TANG, M;WAN, Q;SUN, S;HU, J;SUN, Z;
Journal
China Biotechnology
Title
The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells
Year
2020
Author
TANG, M;WAN, Q;SUN, S;HU, J;SUN, Z;
Journal
China Biotechnology
Title
Progress in the research of p53 tumour suppressor activity controlled by Numb in triple-negative breast cancer
Year
2020
Author
Xian, J;Cheng, Y;Qin, X;Cao, Y;Luo, Y;Cao, Y;
Journal
J. Cell. Mol. Med.
Title
Progress in the research of p53 tumour suppressor activity controlled by Numb in triple-negative breast cancer
Year
2020
Author
Xian, J;Cheng, Y;Qin, X;Cao, Y;Luo, Y;Cao, Y;
Journal
J. Cell. Mol. Med.
Title
BRG1 protects the heart from acute myocardial infarction by reducing oxidative damage through the activation of the NRF2/HO1 signaling pathway
Year
2020
Author
Liu, X;Yuan, X;Liang, G;Zhang, S;Zhang, G;Qin, Y;Zhu, Q;Xiao, Q;Hou, N;Luo, JD;
Journal
Free radical biology & medicine
Title
BRG1 protects the heart from acute myocardial infarction by reducing oxidative damage through the activation of the NRF2/HO1 signaling pathway
Year
2020
Author
Liu, X;Yuan, X;Liang, G;Zhang, S;Zhang, G;Qin, Y;Zhu, Q;Xiao, Q;Hou, N;Luo, JD;
Journal
Free radical biology & medicine