IL-1 alpha/IL1A Antibody Guideline: Preparation, Dilution and Applications

IL-1 alpha/IL1A Antibody Overview

There are 9 IL-1 alpha/IL1A antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, ELISA(Cap), WB, IP. There are 7 IL-1 alpha/IL1A antibody for ELISA, 1 IL-1 alpha/IL1A antibody for IHC-P, 1 IL-1 alpha/IL1A antibody for ELISA(Cap), 1 IL-1 alpha/IL1A antibody for WB, 1 IL-1 alpha/IL1A antibody for IP. Among all these IL-1 alpha/IL1A antibodies, there are 1 anti-IL-1 alpha/IL1A mouse monoclonal antibodies , 3 anti-IL-1 alpha/IL1A rabbit monoclonal antibodies , 5 anti-IL-1 alpha/IL1A rabbit polyclonal antibodies . All the IL-1 alpha/IL1A anbodies are produced in house and all are in stock. IL-1 alpha/IL1A antibody customerized service is available.

IL-1 alpha/IL1A Antibody ELISA

IL-1 alpha/IL1A antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human IL-1 alpha / IL-1F1 / IL1A (rh IL-1 alpha / IL-1F1 / IL1A; Catalog#10128-HNCH; NP_000566.3; Ser113-Ala271).

IL-1 alpha/IL1A antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

IL-1 alpha/IL1A Antibody western blot

IL-1 alpha/IL1A Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse IL1A/IL-1A/IL-1F1 (rM IL1A/IL-1A/IL-1F1; Catalog#50114-MNAE; NP_034684.2; Ser115-Ser270). IL1A/IL-1A/IL-1F1 specific IgG was purified by Mouse IL1A/IL-1A/IL-1F1 affinity chromatography.

IL-1 alpha/IL1A Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

IL-1 alpha/IL1A Antibody immunohistochemistry

IL-1 alpha/IL1A Antibody immunohistochemistry Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse IL1A/IL-1A/IL-1F1, and purified by antigen affinity chromatography.

IL-1 alpha/IL1A Antibody immunohistochemistry Validated

Application IHC
Dilution 1:200-1:1000
Notes  

IL-1 alpha/IL1A Antibody immunoprecipitation

IL-1 alpha/IL1A Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse IL1A/IL-1A/IL-1F1 (rM IL1A/IL-1A/IL-1F1; Catalog#50114-MNAE; NP_034684.2; Ser115-Ser270). IL1A/IL-1A/IL-1F1 specific IgG was purified by Mouse IL1A/IL-1A/IL-1F1 affinity chromatography.

IL-1 alpha/IL1A Antibody immunoprecipitation Validated

Application IP
Dilution 1-2 μL/mg of lysate
Notes