IL-1 beta Antibody Guideline: Preparation, Dilution and Applications

IL-1 beta Antibody Overview

There are 28 IL-1 beta antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, ELISA(Cap), ELISA(Det), WB. There are 25 IL-1 beta antibody for ELISA, 3 IL-1 beta antibody for IHC-P, 1 IL-1 beta antibody for ELISA(Cap), 1 IL-1 beta antibody for ELISA(Det), 4 IL-1 beta antibody for WB. Among all these IL-1 beta antibodies, there are 5 anti-IL-1 beta mouse monoclonal antibodies , 6 anti-IL-1 beta rabbit monoclonal antibodies , 17 anti-IL-1 beta rabbit polyclonal antibodies . All the IL-1 beta anbodies are produced in house and all are in stock. IL-1 beta antibody customerized service is available.

IL-1 beta Antibody ELISA

IL-1 beta Antibody western blot

IL-1 beta Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse IL-1 beta (rM IL-1 beta; Catalog#50101-MNAE; P10749; Val 118-Ser 269). IL-1 beta specific IgG was purified by Mouse IL-1 beta affinity chromatography.

IL-1 beta Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

IL-1 beta Antibody immunohistochemistry

IL-1 beta Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1 beta / IL1B (rh IL-1 beta / IL1B; Catalog#10139-H07E; NP_000567.1; Met1-Ser269). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL-1 beta Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

IL-1 beta antibody citations

Title
miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1
Year
2019
Author
Zhao, H;Wang, Y;Zhang, X;Guo, Y;Wang, X;
Journal
Toxicol. Appl. Pharmacol.
Title
Purified anacardic acids exert multiple neuroprotective effects in pesticide model of Parkinson's disease: in vivo and in silico analysis
Year
2020
Author
Augusto, RL;Mendonça, IP;de Albuquerque Rego, GN;Pereira, DD;da Penha Gonçalves, LV;Dos Santos, ML;de Souza, RF;Moreno, GMM;Cardoso, PRG;de Souza Andrade, D;da Silva-Júnior, JC;Pereira, MC;Peixoto, CA;Medeiros-Linard, CFB;de Souza, IA;Andrade-da-Costa, BLDS;
Journal
IUBMB Life