IL-18 Antibody Guideline: Preparation, Dilution and Applications

IL-18 Antibody Overview

There are 14 IL-18 antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Cap), WB, IP, IHC-P, ELISA(Det). There are 9 IL-18 antibody for ELISA, 2 IL-18 antibody for ELISA(Cap), 5 IL-18 antibody for WB, 3 IL-18 antibody for IP, 1 IL-18 antibody for IHC-P, 1 IL-18 antibody for ELISA(Det). Among all these IL-18 antibodies, there are 3 anti-IL-18 mouse monoclonal antibodies , 4 anti-IL-18 rabbit monoclonal antibodies , 7 anti-IL-18 rabbit polyclonal antibodies . All the IL-18 anbodies are produced in house and all are in stock. IL-18 antibody customerized service is available.

IL-18 Antibody ELISA

IL-18 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL18 / IL-18 / Interleukin 18 (rh IL18 / IL-18 / Interleukin 18; Catalog#10119-H09E; Q14116; Met1-Asp193). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL-18 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

IL-18 Antibody western blot

IL-18 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse IL18 / IL-18 (Catalog#50073-MNAE; P70380; Asn36-Ser192). IL18 / IL-18 specific IgG was purified by Mouse IL18 / IL-18 affinity chromatography.

IL-18 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

IL-18 Antibody immunoprecipitation

IL-18 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human IL-18 (rh IL-18; Catalog#10119-H09E; Q14116; Met1-Asp193). IL-18 specific IgG was purified by Human IL-18 affinity chromatography.

IL-18 Antibody immunoprecipitation Validated

Application IP
Dilution 0.1-0.5 μL/mg of lysate
Notes  

IL-18 antibody citations

Title
Electroacupuncture ameliorates cardiopulmonary bypass induced apoptosis in lung via ROS/Nrf2/NLRP3 inflammasome pathway
Year
2019
Author
Dhar, R;Zhang, L;Li, Y;Rana, MN;Hu, Z;Li, Z;Cui, H;Tang, H;
Journal
Life Sci.