IL-27 Antibody Guideline: Preparation, Dilution and Applications

IL-27 Antibody Overview

There are 7 IL-27 antibodies which are validated in multiple tissues with various applications, including ELISA, WB. There are 7 IL-27 antibody for ELISA, 2 IL-27 antibody for WB. Among all these IL-27 antibodies, there are 1 anti-IL-27 mouse monoclonal antibodies , 1 anti-IL-27 rabbit monoclonal antibodies , 5 anti-IL-27 rabbit polyclonal antibodies . All the IL-27 anbodies are produced in house and all are in stock. IL-27 antibody customerized service is available.

IL-27 Antibody ELISA

IL-27 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-27 (rh IL-27; Catalog#10076-H08S; NP_005746.2 & NP_663634.2; Met1-Lys229 & Phe29-Pro243). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL-27 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

IL-27 Antibody western blot

IL-27 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human IL-27 (rh IL-27; Catalog#10076-H08S; NP_005746.2 & NP_663634.2; Met1-Lys229 & Phe29-Pro243). IL-27 specific IgG was purified by Human IL-27 affinity chromatography.

IL-27 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

IL-27 Antibody immunoprecipitation