IL17RA Antibody Guideline: Preparation, Dilution and Applications

IL17RA Antibody Overview

There are 15 IL17RA antibodies which are validated in multiple tissues with various applications, including WB, ELISA, FCM, Neutralization. There are 2 IL17RA antibody for WB, 8 IL17RA antibody for ELISA, 6 IL17RA antibody for FCM, 1 IL17RA antibody for Neutralization. Among all these IL17RA antibodies, there are 6 anti-IL17RA rabbit polyclonal antibodies , 6 anti-IL17RA mouse monoclonal antibodies , 3 anti-IL17RA rabbit monoclonal antibodies . All the IL17RA anbodies are produced in house and all are in stock. IL17RA antibody customerized service is available.

IL17RA Antibody ELISA

IL17RA antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human IL17RA (rh IL17RA; Catalog#10895-H08H; NP_055154.3; Met 1-Trp 320).

IL17RA antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

IL17RA Antibody western blot

IL17RA Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse IL17RA, and purified by antigen affinity chromatography.

IL17RA Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

IL17RA Antibody flow cytometry

IL17RA Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL17RA (rh IL17RA; Catalog#10895-H08H; NP_055154.3; Met 1-Trp 320). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL17RA Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

IL17RA Antibody Neutralization

IL17RA Antibody Neutralization Preparation

IL17RA Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of IL17Ra neutralizing antibody is measured by its ability to neutralize IL6 secretion induced by human IL17a (12047-HNAE) in the HFF human foreskin fibroblast .The Neutralization Titer (IC50) is typically 0.30-0.70 µg/mL in the presence of 50 ng/mL Recombinant Human IL17a (12047-HNAE) .
Notes  

IL17RA antibody citations

Title
Enzyme-linked antibody aptamer assays based colorimetric detection of soluble fraction of activated leukocyte cell adhesion molecule
Year
2016
Author
Her, J;Jo, H;Ban, C;
Journal
Sensors and Actuators B: Chemical
Title
Th17 Lymphocytes Induce Neuronal Cell Death in a Human iPSC-Based Model of Parkinson's Disease
Year
2018
Author
Sommer, A;Maxreiter, F;Krach, F;Fadler, T;Grosch, J;Maroni, M;Graef, D;Eberhardt, E;Riemenschneider, MJ;Yeo, GW;Kohl, Z;Xiang, W;Gage, FH;Winkler, J;Prots, I;Winner, B;
Journal
Cell Stem Cell