IL1RAP/IL-1RAcP Antibody Guideline: Preparation, Dilution and Applications

IL1RAP/IL-1RAcP Antibody Overview

There are 8 IL1RAP/IL-1RAcP antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB. There are 5 IL1RAP/IL-1RAcP antibody for ELISA, 4 IL1RAP/IL-1RAcP antibody for FCM, 1 IL1RAP/IL-1RAcP antibody for WB. Among all these IL1RAP/IL-1RAcP antibodies, there are 5 anti-IL1RAP/IL-1RAcP mouse monoclonal antibodies , 3 anti-IL1RAP/IL-1RAcP rabbit polyclonal antibodies . All the IL1RAP/IL-1RAcP anbodies are produced in house and all are in stock. IL1RAP/IL-1RAcP antibody customerized service is available.

IL1RAP/IL-1RAcP Antibody ELISA

IL1RAP/IL-1RAcP antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1RAcP / IL-1R3 (rh IL-1RAcP / IL-1R3; Catalog#10121-H08H; NP_002173.1; Met1-Glu359). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.

IL1RAP/IL-1RAcP antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

IL1RAP/IL-1RAcP Antibody western blot

IL1RAP/IL-1RAcP Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human IL-1RAcP / IL-1R3 (rh IL-1RAcP / IL-1R3; Catalog#10121-H08H; NP_002173.1; Met1-Glu359). IL-1RAcP / IL-1R3 specific IgG was purified by Human IL-1RAcP / IL-1R3 affinity chromatography.

IL1RAP/IL-1RAcP Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

IL1RAP/IL-1RAcP Antibody flow cytometry

IL1RAP/IL-1RAcP Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL1R3 / IL1RAP (rh IL1R3; Catalog#10121-H08H; NP_002173.1; Met 1-Glu 359). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL1RAP/IL-1RAcP Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

IL1RAP/IL-1RAcP antibody citations

Title
IL-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia
Year
2015
Author
Southcombe, JH;Redman, CW;Sargent, IL;Granne, I;
Journal
Clin. Exp. Immunol.