IL2 Protein Overview: Sequence, Structure, Function and Protein Interaction

IL2 Protein Overview

IL2 reagents

The action of the T-cell growth factor interleukin-2 (IL2; 147680) requires the presence of a cell surface receptor. As most peripheral as well as thymic T cells do not carry the receptor in vivo, the regulated expression of IL2R appears to be a safeguard against a catastrophic spread of T-cell proliferation by an immunogenic stimulus. The receptor is a heterodimer, consisting of 1 alpha and 1 beta chain; the beta chain (146710) was not characterized until 1989. The receptor molecule, a glycoprotein, has a relative mass of about 55,000. Its intracellular precursor is smaller. Leonard et al. (1983) used a monoclonal antibody for T-cell growth factor to characterize the receptor. Leonard et al. (1984), Nikaido et al. (1984), and Cosman et al. (1984) cloned the IL2R gene. Leonard et al. (1984) identified 1 gene but 2 IL2R mRNAs that differ in their polyadenylation signals. They also isolated an additional cDNA that may correspond to an alternatively spliced mRNA that lacks a 216-bp segment and appears to encode an altered membrane protein that cannot bind interleukin-2. Nikaido et al. (1984) determined the primary structure of the precursor, which has 272 amino acid residues. Hatakeyama et al. (1985) provided evidence that the IL2 receptor is generated, at least in part, by a single gene, namely, the gene encoding Tac antigen (p55). Hatakeyama et al. (1986) used site-directed mutation in the Tac antigen cDNA to generate a mutant receptor. Results of expression studies led them to propose that a molecule that is associated with but distinct from the Tac antigen is involved in the regulation of the functional IL2R complex. Tsudo et al. (1986) arrived at a similar conclusion on the basis of other studies. Robb et al. (1988) employed further cytomutagenesis studies of the Tac molecule to derive a correlation between the structure and function of various components of the molecule. The Tac antigen represents the alpha chain of IL2R, which binds IL2 with low affinity (Leonard et al., 1984; Nikaido et al., 1984), whereas the beta chain by itself does not bind IL2 when expressed in COS-7 cells. However, coexpression of the 2 chains leads to the formation of a high-affinity receptor. The same situation exists with the alpha and beta chains of the GM-CSF receptor (CSF2RA; 306250).

IL2 protein family

Belongs to the IL-2 family.

IL2 protein name

Recommended name
Interleukin-2
Short name
IL-2||TCGF
Aliases
IL-2, T cell growth factor, TCGF
Alternative name
T-cell growth factor INN: Aldesleukin

IL2 Protein Sequence

Species Human IL2 protein
Length 153
Mass (Da) 17628
Sequence Human IL2 protein sequence
Species Mouse IL2 protein
Length 169
Mass (Da) 19400
Sequence Mouse IL2 protein sequence
Species Rat IL2 protein
Length 155
Mass (Da) 17633
Sequence Rat IL2 protein sequence

IL2 Protein Molecular Weight & PI

Interleukin-2 precursor (IL-2) (T-cell growth factor) (TCGF) (Aldesleukin) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 21-153 Interleukin-2.

Molecular weight (Da)

15418.01

Theoretical pI

7.05

IL2 Protein Structure

Interleukin-2 mutant D10
Deposited
2011-01-12   Released:  2012-04-11
Deposition Author(s)
Levin, A.M., Bates, D.L., Ring, A.M., Lin, J.T., Su, L., Krieg, C., Bowman, G.R., Novick, P., Pande, V.S., Khort, H.E., Boyman, O., Fathman, C.G., Garcia, K.C.
Organism(s)
Homo sapiens
Expression System
Trichoplusia ni
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.1000 Å
R-Value Free
0.263
R-Value Work
0.221
3QB1 From PDB

Human IL2 protein Secondary structure

IL2 Protein Interaction

Recombinant IL2 Protein Feature

IL2 Protein, Human, Recombinant (L38M, L39S, C145A)

High Purity
> 85 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.
High Activity
Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is typically 0.35-1.4 ng/mL.

Recombinant IL2 protein citations

Title
A chemically-defined trifunctional antibody-cytokine-drug conjugate with potent anti-tumor activity
Year
2014
Author
T List, G Casi, D Neri
Journal
Cancer research
Title
Generation of an immortalized mesenchymal stem cell line producing a secreted biosensor protein for glucose monitoring
Year
2017
Author
Siska, EK;Weisman, I;Romano, J;Ivics, Z;Izsvák, Z;Barkai, U;Petrakis, S;Koliakos, G;
Journal
PLoS ONE
Title
Filgrastim enhances T-cell clearance by antithymocyte globulin exposure after unrelated cord blood transplantation
Year
2018
Author
de Koning, C;Gabelich, JA;Langenhorst, J;Admiraal, R;Kuball, J;Boelens, JJ;Nierkens, S;
Journal
Blood Adv
Title
NK cell-mediated anti-leukemia cytotoxicity is enhanced using a NKG2D ligand MICA and anti-CD20 scfv chimeric protein
Year
2018
Author
Zou, Y;Luo, W;Guo, J;Luo, Q;Deng, M;Lu, Z;Fang, Y;Zhang, CC;
Journal
Eur. J. Immunol.
Title
Utilizing VEGF165b mutant as an effective immunization adjunct to augment antitumor immune response
Year
2019
Author
Zhang, H;Jia, E;Xia, W;Lv, T;Lu, C;Xu, Z;Zhu, W;
Journal
Vaccine
Title
CD137 ligand feedback upregulates PD-L1 expression on lung cancer via T cell production of IFN-γ
Year
2019
Author
Wang, H;Yan, Z;Hao, J;Yang, B;Wang, J;Yi, L;Wang, X;Li, S;Zhang, H;Zhang, S;
Journal
Thorac Cancer
Title
TGFβ inhibition via CRISPR promotes the long-term efficacy of CAR-T cells against solid tumors
Year
2020
Author
Tang, N;Cheng, C;Zhang, X;Qiao, M;Li, N;Mu, W;Wei, XF;Han, W;Wang, H;
Journal
JCI Insight

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