IL20RA Protein

IL20RA Protein Overview

IL20RA reagents

Blumberg et al. (2001) identified the receptor for interleukin-20 (IL20; 605619) as a heterodimer of 2 orphan class II cytokine receptor subunits, IL20RA, also called ZCYTOR7, and IL20RB (605621), also called DIRS1. Binding assays using radiolabeled ligand demonstrated that IL20 bound to BHK transfectants expressing both IL20RA and IL20RB, but not to untransfected cells nor to transfectants expressing either receptor subunit alone. Binding of (125)I-labeled IL20 was eliminated in the presence of 100-fold excess of unlabeled IL20 but not in the presence of 100-fold excess of the unrelated cytokine, IL21 (605384). The binding data revealed 88,000 IL20 receptors per cell, with a binding affinity of approximately 1.5 nM. Both receptor subunits were expressed in skin and were dramatically upregulated in psoriatic skin. By receptor binding analysis, Wang et al. (2002) showed that human IL24 (604136) interacted with both IL20RA/IL20RB and IL22RA (605457)/IL20RB heterodimers. Based on phylogenetic analysis, Sheikh et al. (2004) hypothesized that IL10RB (123889) and either IL20RA or IL22RA1 form the heterodimer that serves as the IL26 (605679) receptor. Both IL20RA and IL22RA1 activate STAT3 (102582) upon ligand binding, but in conjunction with a chimeric IFNGR1 (107470) intracellular chain, they activate STAT1 (600555). EMSA analysis demonstrated that IL26 induced STAT1 activation in transfected cell lines expressing IL10RB and IL20RA/IFNGR1 chimeras, but not in cell lines expressing IL10RB and IL22RA1/IFNGR1 chimeras. In the absence of the IFNGR1 intracellular chain, STAT3 was activated predominantly. STAT1 activation was inhibited in the presence of antibodies to IL10RB, IL20RA, or IL26. ELISA and chromatographic analysis indicated that IL26 interacted predominantly with IL20RA. Sheikh et al. (2004) showed that IL20RA has a restricted tissue distribution, in contrast with the ubiquitous expression of IL10RB. IL26 stimulation of a colorectal adenocarcinoma cell line expressing IL10RB and IL20RA consistently induced stronger activation of STAT3 than STAT1. Sheikh et al. (2004) concluded that the receptor combination of IL10RB and IL20RA is unique and specific for IL26.

IL20RA protein family

Belongs to the type II cytokine receptor family.

IL20RA protein name

Recommended name
Interleukin-20 receptor subunit alpha
Short name
IL-20 receptor subunit alpha||IL-20R-alpha||I
Aliases
IL-20R1, ZCYTOR7
Alternative name
Cytokine receptor class-II member 8 Cytokine receptor family 2 member 8 IL-20R1 ZcytoR7

IL20RA Protein Sequence

Species Human IL20RA protein
Length 553
Mass (Da) 62485
Sequence Human IL20RA protein sequence
Species Mouse IL20RA protein
Length 546
Mass (Da) 61978
Sequence Mouse IL20RA protein sequence
Species
Length
Mass (Da)
Sequence

IL20RA Protein Molecular Weight & PI

Interleukin-20 receptor subunit alpha precursor (IL-20 receptor subunit alpha) (IL-20R-alpha) (IL-20RA) (Cytokine receptor class-II member 8) (Cytokine receptor family 2 member 8) (CRF2-8) (IL-20R1) (ZcytoR7) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 30-553 Interleukin-20 receptor subunit alpha.

Molecular weight (Da)

59375.59

Theoretical pI

4.67

IL20RA Protein Structure

IL20/IL201/IL20R2 Ternary Complex
Deposited
2012-02-09   Released:  2012-07-18
Deposition Author(s)
Logsdon, N.J., Walter, M.R.
Organism(s)
Homo sapiens
Expression System
Drosophila
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.8000 Å
R-Value Free
0.278
R-Value Work
0.230
4DOH From PDB

Human IL20RA protein Secondary structure

IL20RA Protein Interaction

Recombinant IL20RA Protein Feature

IL20RA Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

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