Intrinsic Factor Antibody Guideline: Preparation, Dilution and Applications

Intrinsic Factor Antibody Overview

There are 5 Intrinsic Factor antibodies which are validated in multiple tissues with various applications, including FCM, ICC/IF, WB, IP, ELISA, ELISA(Cap), IHC-P. There are 1 Intrinsic Factor antibody for FCM, 1 Intrinsic Factor antibody for ICC/IF, 1 Intrinsic Factor antibody for WB, 1 Intrinsic Factor antibody for IP, 2 Intrinsic Factor antibody for ELISA, 1 Intrinsic Factor antibody for ELISA(Cap), 1 Intrinsic Factor antibody for IHC-P. Among all these Intrinsic Factor antibodies, there are 3 anti-Intrinsic Factor rabbit monoclonal antibodies , 2 anti-Intrinsic Factor rabbit polyclonal antibodies . All the Intrinsic Factor anbodies are produced in house and all are in stock. Intrinsic Factor antibody customerized service is available.

Intrinsic Factor Antibody ELISA

Intrinsic Factor antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; Catalog#13544-H08H; P27352-1; Met1-Tyr417).

Intrinsic Factor antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

Intrinsic Factor Antibody western blot

Intrinsic Factor Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; Catalog#13544-H08H; P27352-1; Met1-Tyr417).

Intrinsic Factor Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Intrinsic Factor Antibody flow cytometry

Intrinsic Factor Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; Catalog#13544-H08H; P27352-1; Met1-Tyr417).

Intrinsic Factor Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

Intrinsic Factor Antibody immunohistochemistry

Intrinsic Factor Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human GIF / Gastric intrinsic factor (rh GIF / Gastric intrinsic factor; Catalog#13544-H08H; P27352-1; Met1-Tyr417). GIF / Gastric intrinsic factor specific IgG was purified by Human GIF / Gastric intrinsic factor affinity chromatography.

Intrinsic Factor Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

Intrinsic Factor Antibody immunoprecipitation

Intrinsic Factor Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; Catalog#13544-H08H; P27352-1; Met1-Tyr417).

Intrinsic Factor Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

Intrinsic Factor Antibody ICC / IF

Intrinsic Factor Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; Catalog#13544-H08H; P27352-1; Met1-Tyr417).

Intrinsic Factor Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes