LIF Protein Overview: Sequence, Structure, Function and Protein Interaction

LIF Protein Overview

By use of the murine cDNA of the recently cloned murine leukemia-inhibitory factor (LIF) gene as a hybridization probe, Gough et al. (1988) isolated the human homolog from a genomic library. The nucleotide sequence of the human gene indicated 78% sequence identity with murine LIF, with no insertions or deletions. Williams et al. (1988) proposed that LIF is identical to a differentiation inhibitory activity (DIA) in embryonic stem (ES) cells.

LIF protein family

Belongs to the LIF/OSM family.

LIF protein name

Recommended name
Leukemia inhibitory factor
Short name
LIF||D factor||MLPLI
CDF, cholinergic differentiation factor, DIA, differentiation inhibitory activity, differentiation-inducing factor, hepatocyte-stimulating factor III, HILDA, human interleukin in DA cells
Alternative name
Differentiation-stimulating factor Melanoma-derived LPL inhibitor INN: Emfilermin

LIF Protein Sequence

Species Human LIF protein
Length 202
Mass (Da) 22008
Sequence Human LIF protein sequence
Species Mouse LIF protein
Length 203
Mass (Da) 22287
Sequence Mouse LIF protein sequence
Species Rat LIF protein
Length 202
Mass (Da) 22113
Sequence Rat LIF protein sequence

LIF Protein Molecular Weight & PI

Leukemia inhibitory factor precursor (LIF) (Differentiation-stimulating factor) (D factor) (Melanoma-derived LPL inhibitor) (MLPLI) (Emfilermin) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 23-202 Leukemia inhibitory factor.

Molecular weight (Da)


Theoretical pI


LIF Protein Structure

Crystal structure of leukemia inhibitory factor in complex with gp130
2003-06-27   Released:  2003-10-14
Deposition Author(s)
Boulanger, M.J., Bankovich, A.J., Kortemme, T., Baker, D., Garcia, K.C.
Homo sapiens
Expression System
Spodoptera frugiperda
Experimental Data Snapshot
2.5000 Å
R-Value Free
R-Value Work

Human LIF protein Secondary structure

LIF Protein Interaction

Recombinant LIF Protein Feature

LIF Protein, Mouse, Recombinant

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.

Recombinant LIF protein citations

Sema3A and HIF1α co-overexpressed iPSC-MSCs/HA scaffold facilitates the repair of calvarial defect in a mouse model
Li, J;Wang, T;Li, C;Wang, Z;Wang, P;Zheng, L;
J. Cell. Physiol.
Semaphorin 3A-hypoxia inducible factor 1 subunit alpha co-overexpression enhances the osteogenic differentiation of induced pluripotent stem cells-derived mesenchymal stem cells in vitro
Li, JY;Wang, TT;Li, C;Wang, ZF;Li, S;Ma, L;Zheng, LL;
Chin. Med. J.

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