NPC2 Antibody Guideline: Preparation, Dilution and Applications

NPC2 Antibody Overview

There are 12 NPC2 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, WB, IP, ELISA(Det), ELISA(Cap). There are 8 NPC2 antibody for ELISA, 4 NPC2 antibody for IHC-P, 2 NPC2 antibody for WB, 1 NPC2 antibody for IP, 1 NPC2 antibody for ELISA(Det), 1 NPC2 antibody for ELISA(Cap). Among all these NPC2 antibodies, there are 2 anti-NPC2 mouse monoclonal antibodies , 6 anti-NPC2 rabbit monoclonal antibodies , 4 anti-NPC2 rabbit polyclonal antibodies . All the NPC2 anbodies are produced in house and all are in stock. NPC2 antibody customerized service is available.

NPC2 Antibody ELISA

NPC2 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NPC2 (rh NPC2; Catalog#13341-H08H; NP_006423.1; Met1-Leu151). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

NPC2 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

NPC2 Antibody western blot

NPC2 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Rat NPC2 (rR NPC2; Catalog#80706-R08H; CAD56199.1; Met1-Gly152). NPC2 specific IgG was purified by Rat NPC2 affinity chromatography.

NPC2 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

NPC2 Antibody immunohistochemistry

NPC2 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human Niemann-Pick disease type C2 / NPC2 (rh Niemann-Pick disease type C2 / NPC2; Catalog#13341-H08H; NP_006423.1; Met1-Leu151). Niemann-Pick disease type C2 / NPC2 specific IgG was purified by Human Niemann-Pick disease type C2 / NPC2 affinity chromatography.

NPC2 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:10000-1:20000
Notes  

NPC2 Antibody immunoprecipitation

NPC2 Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human NPC2 (rh NPC2; Catalog#13341-H08H; NP_006423.1; Met1-Leu151).

NPC2 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes