NPC2 Protein Overview: Sequence, Structure, Function and Protein Interaction

NPC2 Protein Overview

Kirchhoff et al. (1990) originally cloned HE1 by differential screening of a human epididymis cDNA library. Krull et al. (1993) found HE1 to be highly expressed in all parts of the epididymis. Kirchhoff et al. (1996) cloned the full-length HE1 cDNA and found that it corresponds to a single copy gene which is well-conserved among mammals. The 151-amino acid HE1 glycoprotein has a molecular mass of 25 to 27 kD and contains a 19-amino acid signal that, along with homologs from numerous mammalian species, represents a major secretory component of the epididymal fluid. By Northern blot analysis, Naureckiene et al. (2000) detected a single transcript of 0.9 kb in all tissues examined, with highest levels in testis, kidney, and liver and lowest levels in lung and muscle. Subcellular fractionation of rat liver showed that HE1 is a lysosomal protein.

NPC2 protein family

Belongs to the NPC2 family.

NPC2 protein name

Recommended name
NPC intracellular cholesterol transporter 2
EDDM1, epididymal protein 1, HE1, NP-C2
Alternative name
Epididymal secretory protein E1 Human epididymis-specific protein 1 Niemann-Pick disease type C2 protein

NPC2 Protein Sequence

Species Human NPC2 protein
Length 151
Mass (Da) 16570
Sequence Human NPC2 protein sequence
Species Mouse NPC2 protein
Length 149
Mass (Da) 16442
Sequence Mouse NPC2 protein sequence
Mass (Da)

NPC2 Protein Molecular Weight & PI

NPC intracellular cholesterol transporter 2 precursor (Epididymal secretory protein E1) (Human epididymis-specific protein 1) (He1) (Niemann-Pick disease type C2 protein) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 20-151 NPC intracellular cholesterol transporter

Molecular weight (Da)


Theoretical pI


NPC2 Protein Structure

Structure of human NPC1 middle lumenal domain bound to NPC2
2016-07-19   Released:  2016-08-24
Deposition Author(s)
Li, X.
Homo sapiens
Expression System
Bacteria, Homo sapiens
Experimental Data Snapshot
2.4050 Å
R-Value Free
R-Value Work

Human NPC2 protein Secondary structure

NPC2 Protein Interaction

Recombinant NPC2 Protein Feature

NPC2 Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

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