RANKL Antibody Guideline: Preparation, Dilution and Applications

RANKL Antibody Overview

There are 8 RANKL antibodies which are validated in multiple tissues with various applications, including ELISA, Neutralization, WB, ELISA(Det), IP, ELISA(Cap). There are 5 RANKL antibody for ELISA, 1 RANKL antibody for Neutralization, 3 RANKL antibody for WB, 1 RANKL antibody for ELISA(Det), 1 RANKL antibody for IP, 1 RANKL antibody for ELISA(Cap). Among all these RANKL antibodies, there are 1 anti-RANKL mouse monoclonal antibodies , 4 anti-RANKL rabbit monoclonal antibodies , 3 anti-RANKL rabbit polyclonal antibodies . All the RANKL anbodies are produced in house and all are in stock. RANKL antibody customerized service is available.

RANKL Antibody ELISA

RANKL Antibody western blot

RANKL Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human RANKL / OPGL / TNFSF11 / CD254 (rh RANKL / OPGL / TNFSF11 / CD254; Catalog#11682-H15H; O14788-2; Gly 63-Asp 244).

RANKL Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

RANKL Antibody immunoprecipitation

RANKL Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human RANKL / OPGL / TNFSF11 / CD254 (rh RANKL / OPGL / TNFSF11 / CD254; Catalog#11682-HNCH; O14788-2; Gly63-Asp244).

RANKL Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

RANKL Antibody Neutralization

RANKL Antibody Neutralization Preparation

RANKL Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of TNFSF11 Neutralizing Antibody is Measured by its ability to neutralize TNFSF11 induced osteoclast-like cell formation in RAW 264.7 mouse macrophages. The Neutralization titer (IC50) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human RANKL/OPGL/TNFSF11/CD254.
Notes