TIM-3 Antibody Guideline: Preparation, Dilution and Applications

TIM-3 Antibody Overview

There are 12 TIM-3 antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Det), ELISA(Cap), WB. There are 12 TIM-3 antibody for ELISA, 1 TIM-3 antibody for ELISA(Det), 1 TIM-3 antibody for ELISA(Cap), 2 TIM-3 antibody for WB. Among all these TIM-3 antibodies, there are 2 anti-TIM-3 mouse monoclonal antibodies , 3 anti-TIM-3 rabbit monoclonal antibodies , 7 anti-TIM-3 rabbit polyclonal antibodies . All the TIM-3 anbodies are produced in house and all are in stock. TIM-3 antibody customerized service is available.

TIM-3 Antibody ELISA

TIM-3 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TIMD3 (rh TIMD3; Catalog#10390-H08H; NP_116171.3; Met 1-Arg 200). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .

TIM-3 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

TIM-3 Antibody western blot

TIM-3 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse TIM-3 / HAVCR2 (Catalog#51152-M08H; AAL65156.1; Met1-Arg191). TIM-3 / HAVCR2 specific IgG was purified by Mouse TIM-3 / HAVCR2 affinity chromatography.

TIM-3 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

TIM-3 Antibody immunoprecipitation

TIM-3 antibody citations

Title
Soluble Tim3 detection by time-resolved fluorescence immunoassay and its application in membranous nephropathy
Year
2020
Author
Chen, M;Wang, L;Wang, Y;Zhou, X;Liu, X;Chen, H;Huang, B;Hu, Z;
Journal
J. Clin. Lab. Anal.
Title
Soluble Tim3 detection by time-resolved fluorescence immunoassay and its application in membranous nephropathy
Year
2020
Author
Chen, M;Wang, L;Wang, Y;Zhou, X;Liu, X;Chen, H;Huang, B;Hu, Z;
Journal
J. Clin. Lab. Anal.